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物种
Human分子别名
GUC2C, STAR, STA receptor, hSTAR, GC-CAccession
P25092表达序列
Ser24-Gln430, with C-hIgG1 Fc and Avitag表达宿主
HEK293分子量
95-110kDa (Reducing)
纯度
＞95% by SDS-PAGE内毒素含量
<1EU/μg性状
Lyophilized Powder缓冲体系
PBS, pH7.4.溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1. Michael S. Magee,Crystal L. Kraft,Tara S. Abraham,Trevor R. Baybutt,Glen P. Marszalowicz,Peng Li: GUCY2C-directed CAR-T cells oppose colorectal cancer metastases without autoimmunity. OncoImmunology, Volume 5, 2016 - Issue 10.
Guanylyl Cyclase C (GUCY2C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (GC) family expressed by intestinal epithelial cells from the duodenum to rectum. Mature human GUCY2C consists of an extracellular domain (ECD) with a ligand binding domain, a transmembrane segment and a cytoplasmic region possessing a kinase and GC catalytic domain. Endogenous ligands of GUCY2C include guanylin and uroguanylin. GUCY2C in epithelial cells plays an important role in cell dynamics and homeostatic balance of proliferation, metabolism, and differentiation that organizes the guanylyl cyclase C hormone axis. GUCY2C is also expressed in the brain and is implicated in attention deficiency and hyperactive behavior. The activation of cyclic guanosine monophosphate (cGMP), a second intracellular messenger, can stimulate the production of cyclic guanosine monophosphate (CGMP), which plays a role in regulating homeostasis, maintaining intestinal barrier function, and exerting anti-inflammatory activity.
电泳(SDS-PAGE)
1μg (R: reducing condition, N: non-reducing condition).
ELISA
Immobilized Anti-Human GUCY2C (Indusatumab) at 0.5μg/mL (100μL/well) can bind Biotinylated Guanylyl Cyclase C/GUCY2C Fc&Avi Tag Protein, Human (Cat. No. UA010880) with EC50 of 3.98-7.82ng/mL.
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