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Custom murine mAb

STARTER customized mouse monoclonal antibody was prepared using hybridoma, and the specified antibody was purified from mouse ascites or purified by antibody recombinant expression and purification technology. The way of hybridoma producing McAb is more mature than rabbit McAb. The mouse strain is more stable after verification, and the immune response is clear.

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Service Process

  • Antigen Preparing

  • Immunization

  • Serum Screening and Test

  • Hybridoma Preparing and Screening

  • Ascites Preparation and Purification

    Antibody Expression and Purification

  • Validation

Service Details

STEPS Details TAT(WEEKS)
Antigen Preparing 3.5mg Antigen 4-5
Immunization 5 Mice 9-11
Serum Screening and Test ELISA ScreeningAffinity Ranking 1-2
Hybridoma Preparing and Screening Cell Fusion and Screening
Two rounds of Subcloning
2
Ascites Preparation and Purification Cell Injecting Ascites Collecting
Antibody purification
2
Antibody Expression & Purification Construction of Expression Vector
Transient Expression
Antibody Purification
2-3
Validation

Antibody Titer>1:50,000

Additional verifications as requested by customer warrants positivity

2
Deliverables Purified Antibody 20-25

Remark (cost additional):

On the basis of the standard package, the customer has the option to increase the number of immunised animals and antigens;

Optional serum return;

May add specified QC assays。

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