产品中心 ELISA试剂盒 OneStep ELISA 试剂盒
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抗原名称
Serum Albumin免疫原
Recombinant Protein抗体类型
Recombinant mAb反应种属 ?
Hu纯化方式
Protein A储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
Precision
Intra-assay: 3.7%;
Inter-assay: 7.4%Sample type
Cell culture supernatant, Serum,PlasmaAssay type
Competitive (quantitative)Sensitivity
3.56 ng/mlRange
39.06 ng/mL – 2500 ng/mLRecovery
Cell culture supernatant: 100%
Serum: 95%
Plasma: 96%Assay time
60 minutesSpecies reactivity
Human
Human Serum Albumin (HSA) is a single polypeptide chain composed of 585 amino acids, with a molecular weight of ~66.5 kDa. It is rich in cysteine residues (17 disulfide bonds) and lacks carbohydrates, making it highly soluble and stable. The protein folds into a heart-shaped tertiary structure with three homologous domains (I, II, and III), each divided into two subdomains (A and B). The structure is stabilized by disulfide bonds and hydrophobic interactions. HSA has multiple ligand-binding sites, notably for fatty acids, hormones, and drugs, located in hydrophobic pockets and flexible loops. HAS is a vital multifunctional plasma protein critical for homeostasis. It maintains ~80% of plasma colloid osmotic pressure to prevent vascular fluid leakage, transports hydrophobic molecules (e.g., fatty acids, hormones, drugs) and metal ions (Ca²⁺, Cu²⁺), and exerts antioxidant activity via its Cys-34 thiol group to neutralize free radicals and bind pro-oxidant metals. HSA also buffers blood pH using histidine residues and supports endothelial stability by regulating vascular permeability and nitric oxide bioavailability, thereby modulating blood flow. These roles collectively highlight HSA’s indispensable contribution to systemic health and disease regulation. HSA exhibits significant evolutionary conservation, sharing high sequence homology with serum albumin in other mammals. For instance, it shows ~76% sequence identity with Bovine Serum Albumin (BSA) and ~78% identity with Mouse Serum Albumin, highlighting its structural and functional preservation across species. This homology extends to its three-domain architecture (domains I, II, and III) and the characteristic disulfide bond patterns, both of which are remarkably conserved. low levels, caused by reduced synthesis (e.g., liver cirrhosis), excessive loss (e.g., nephrotic syndrome), malnutrition, or inflammation (e.g., sepsis), leads to edema, ascites, impaired drug efficacy, and poorer outcomes in critical illnesses. Conversely, elevated levels, typically due to dehydration (hemoconcentration) or rare genetic factors, is often transient but may signal chronic inflammation or metabolic disorders if persistently elevated. Applications of the Human Serum Albumin Competitive OneStep ELISA Kit: Human Serum Albumin Competitive OneStep ELISA kit enables rapid, accurate quantification of Serum Albumin in serum, plasma, or cell culture supernatants. Advantages of this Competitive OneStep ELISA Kit Design: Simplified Workflow: Combines sample incubation and detection in a single step, eliminating intermediate washing and reducing hands-on time. High Efficiency: Achieves results within 1 hours, ideal for high-throughput studies or urgent diagnostics. Enhanced Precision: Minimizes procedural variability and cross-contamination risks through optimized reagent formulation. Broad Compatibility: Validated for diverse biological matrices, ensuring flexibility in experimental design. This kit’s streamlined protocol and robust performance make it an indispensable tool for researchers and clinicians seeking reliable Serum Albumin quantification in time-sensitive or resource-limited settings.
ELISA
Standard curve
Example of Human Serum Albumin standard curve in Assay Diluent #1.Linearity
The concentrations of Human Serum Albumin were measured and interpolated from the target standard curves and corrected for sample dilution.
The sample is undiluted samples are as follows: Human serum was 5×10^4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 42.33 mg/mL in human serum.Linearity
The sample is undiluted samples are as follows: Human plasma was 5×10^4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 38.80 mg/mL in human plasma.Protocol Diagram
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