产品中心 ELISA试剂盒 OneStep ELISA 试剂盒
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抗原名称
M13 Phage免疫原
Recombinant Protein抗体类型
Recombinant mAb反应种属 ?
Others纯化方式
Protein A储存条件
2 to 8 °C as supplied.
Precision
板内变异系数 (Intra-assay): 6.1%;
板间变异系数 (Inter-assay): 7.5%Sample type
Cell culture supernatantAssay type
Sandwich (quantitative)Sensitivity
7.079E+03 particles /mlRange
2.205E+04 particles /ml – 2.500E+06 particles /mlRecovery
Cell culture supernatant: 92%Assay time
60 minutes
M13 is a filamentous phage that has attracted much attention in the field of molecular biology and biotechnology. It belongs to the family Inoviridae and mainly infects Escherichia coli (E. coli). M13 phage has a simple single-stranded DNA genome of about 6.4 kilobases and a rod-shaped morphology. M13 phage can display foreign peptides or proteins on its surface. By genetically engineering the M13 genome to include genes encoding proteins of interest in the phage coat protein genes, phage particles are generated that can display proteins on their surface. Phage titration ELISA is a technique used to determine the concentration or titer of phage (bacteriophage) particles in a given sample. The Phage Titration ELISA determines the relative number of phage particles and provides a rapid, sensitive and reproducible alternative method for titrating M13 phage preparations.
ELISA
Standard curve
Example of M13 standard curve in Assay Diluent #1.Leading Competitor comparison
Protocol Diagram
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