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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Mouse IgM OneStep ELISA Kit

货号： S0C3022

Datasheet COA
  • 价格： ￥3,000
  • 规格：
  • 数量：
斯达特大包装询价 大包装询价

产品介绍 评论(0)

产品规格

  • 抗原名称

    IgM

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Ms

  • 纯化方式

    Protein A

  • 储存条件

    2 to 8 °C as supplied.

  • 稀释度

    [{"application": "ELISA", "dilution": "", "species": ""}, {"application": "Sandwich ELISA", "dilution": "", "species": ""}, {"application": "CLIA", "dilution": "", "species": ""}, {"application": "Lateral Flow", "dilution": "", "species": ""}, {"application": "Dot Blot", "dilution": "", "species": ""}, {"application": "WB", "dilution": "", "species": ""}, {"application": "IP", "dilution": "", "species": ""}, {"application": "IHC-P", "dilution": "", "species": ""}, {"application": "ICC", "dilution": "", "species": ""}, {"application": "IF", "dilution": "", "species": ""}, {"application": "ICFCM", "dilution": "", "species": ""}, {"application": "FCM", "dilution": "", "species": ""}, {"application": "mIHC", "dilution": "", "species": ""}]
试剂盒参数

    • Precision

      Intra-assay: 3.2%；

      Inter-assay: 6.8%

    • Sample type

      Serum, Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      0.298 ng/ml

    • Range

      1.25 ng/mL - 80 ng/mL

    • Recovery

      Serum 92%
      Plasma 102%

    • Assay time

      60 minutes

    • Species reactivity

      Mouse

    • CROSS REACTIVITY

      /

    • INTERFERENCE

      /

    • NIBSC convert

      /
背景介绍

  • Mouse immunoglobulin M (IgM) is a crucial component of the humoral immune system and is the first antibody produced by B-cells in response to an antigenic challenge. It exists in two forms: a pentameric (19S) form found in serum and a monomeric (8S) form on the surface of lymphocytes. The IgM pentamer, which is the predominant form in circulation, consists of five monomeric units and has a molecular weight of approximately 900 kDa, while the hexameric form has a molecular weight of about 1050 kDa. Each monomer of IgM is composed of two heavy chains and two light chains, with the heavy chain having four constant domains (CH1-CH4) and the light chain having one constant domain (CL). The CH4 domain facilitates oligomerization, leading to the formation of penta- or hexamers, and these oligomers are covalently linked via disulfide bonds, resembling a snowflake structure. A joining (J) chain, a 15 kDa polypeptide, is essential for the secretion of IgM into mucosal areas.

  • ELISA

    • Standard curve
      Example of Mouse IgM standard curve in Assay Diluent #1.

    • Linearity
      The concentrations of Mouse IgM were measured and interpolated from the target standard curves and corrected for sample dilution.
      #1 sample is  undiluted samples are as follows:  Mouse serum was 4×10^3 fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 262.88 ug/mL in Mouse serum.
      #2 sample is  undiluted samples are as follows:  Mouse plasma was 4×10^3 fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 335.85 ug/mL in Mouse plasma.

    • Leading Competitor comparison

    • Protocol Diagram

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