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抗原名称
His tag免疫原
Recombinant Protein抗体类型
Recombinant mAb反应种属 ?
Species Independent纯化方式
Protein A储存条件
2 to 8 °C as supplied.
Sample type
N-terminal/C-terminal/internal His-tagged proteins.
Mammalian and bacteria cell lysates or cell supernatant.Assay type
Competitive (quantitative)Sensitivity
1 ng/mlRange
1 ng/ml -3160 ng/mlAssay time
60 minutes
A polyhistidine-tag is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein.The total number of histidine residues may vary in the tag from as low as two, to as high as 10 or more His residues. Histidine (His)-tag is widely used for affinity purification of recombinant proteins, but the yield and purity of expressed proteins are quite different. This kit is developed for rapid and high throughput detection of His-tagged proteins.
ELISA
Standard curve
Example of His tag protein (molecular weight of 20 kDa) standard curve in Assay Diluent #1.Standard curve
Example of His tag protein (molecular weight of 20 kDa) standard curve in Assay Diluent #1.Reagent Compatibility
Protocol Diagram
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