产品中心 ELISA试剂盒 OneStep ELISA 试剂盒
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抗原名称
IL-6免疫原
Recombinant Protein抗体类型
Recombinant mAb反应种属 ?
Hu纯化方式
Protein A储存条件
2 to 8 °C as supplied.
Precision
Intra-assay: 5.2%；
Intra-assay: 6.0%
Sample type
Cell culture supernatant, Serum, PlasmaAssay type
Sandwich (quantitative)Sensitivity
0.14 pg/ml
Range
2.1 pg/ml - 136 pg/ml
Recovery
Cell culture supernatant 90%
Serum 98%
Plasma 97%Assay time
60 minutesSpecies reactivity
HumanCROSS REACTIVITY
/INTERFERENCE
/NIBSC convert
The dose response curve of the International Standard (21/308) parallels the OneStep standard curve. To convert sample values obtained with the kit to approximate NIBSC (21/308) units, use the following equation: NIBSC (21/308) approximate value (IU/mL) = 0.142 x OneStep Human IL-6 value (pg/mL).
Interleukin 6 (IL-6) is a prototypical cytokine for maintaining homeostasis. When homeostasis is disrupted by infections or tissue injuries, IL-6 is produced immediately and contributes to host defense against such emergent stress through activation of acute-phase and immune responses. However, dysregulated excessive and persistent synthesis of IL-6 has a pathological effect on, respectively, acute systemic inflammatory response syndrome and chronic immune-mediated diseases.
ELISA
Protocol Diagram
Standard curve
Example of human IL-6 standard curve in Assay Diluent #1.
Standard curve
Example of human IL-6 standard curve in Assay Diluent #1S.
Linearity
The concentrations of IL-6 were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: human PBMC cells stimulated with 10ug/ml PHA for 5days (0.5%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 88,750 pg/mL in stimulated human PBMC supernatant.
#2 sample is undiluted samples are as follows: human PBMC cells culture for 5days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 46 pg/mL in unstimulated human PBMC supernatant.
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