产品中心 ELISA试剂盒 OneStep ELISA 试剂盒
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抗原名称
IL-13免疫原
Recombinant Protein抗体类型
Recombinant mAb反应种属 ?
Mk, Hu纯化方式
Protein A储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
Precision
Intra-assay: 3.8%；
Inter-assay: 4.1%
Sample type
Cell culture supernatant, Serum, PlasmaAssay type
Sandwich (quantitative)Sensitivity
0.3 pg/mlRange
3.9 pg/ml - 250 pg/mlRecovery
Cell culture supernatant 80%
Serum 90%
Plasma 105%Assay time
60 minutesSpecies reactivity
HumanCROSS REACTIVITY
/INTERFERENCE
/NIBSC convert
/
IL-13 is a 132-amino-acid nonglycosylated cytokine that shares common features with IL-4. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils. IL-13 signals through a receptor complex containing IL-13 R alpha 1 and IL-4 R alpha. This complex also functions as the type 2 IL-4 receptor. IL-13 bound IL-13 R alpha 2 with high affinity and can directly promote tumor cell invasiveness and the development of tissue fibrosis. Nevertheless, like IL-4, IL-13 inhibits macrophage production of TNF, IL-1β, and pro-inflammatory chemokines, but can upregulate the synthesis of IL-12 by DCs and macrophages.
ELISA
Standard curve
Example of human IL-13 standard curve in Assay Diluent #1.Linearity
The concentrations of IL-13 were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: human PBMC cells stimulated with 10ug/ml PHA for 5days (10%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 730 pg/mL in stimulated human PBMC supernatant.
#2 sample is undiluted samples are as follows: human PBMC cells culture for 5days (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 16 pg/mL in unstimulated human PBMC supernatant.Leading Competitor comparison
Protocol Diagram
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