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抗原名称
IL-2免疫原
Recombinant Protein抗体类型
Recombinant mAb反应种属 ?
Hu纯化方式
Protein A储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
Precision
Intra-assay: 5.9%；
Inter-assay: 8.5%Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich (quantitative)Sensitivity
5 pg/mlRange
15.6 pg/ml - 1000 pg/mlRecovery
Cell culture supernatant 112% ; Serum 103%; Plasma 118%
Assay time
60 minutesSpecies reactivity
humanCROSS REACTIVITY
/INTERFERENCE
/NIBSC convert
The dose response curve of the International Standard (86/500) parallels the OneStep standard curve. To convert sample values obtained with the kit to approximate NIBSC (86/500) units, use the following equation: NIBSC (86/500) approximate value (IU/mL) = 0.014 x OneStep Human IL-2 value (pg/mL).
Interleukin-2 (IL-2) is an interleukin, a type of cytokine immune system signaling molecule, which is a leukocytotropic hormone that is instrumental in the body natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes, the cells that are responsible for immunity. Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. IL-2 is also necessary during T cell development in the thymus for the maturation of a unique subset of T cells that are termed regulatory T cells (T-Regs). After exiting from the thymus, T-Regs function to prevent other T cells from recognizing and reacting against "self-antigens", which could result in "autoimmunity". T-Regs do this by preventing the responding cells from producing IL-2. Thus, IL-2 is required to discriminate between self and non-self, another one of the unique characteristics of the immune system.
ELISA
Standard curve
Example of human IL-2 standard curve in Assay Diluent #1.
Standard curve
Example of NIBSC (86/500) standard curve in Assay Diluent #1. Human IL-2 standard were calibrated by NIBSC (86/500) with a calibration factor (CF) of 1.4. Human IL-2 standard concentration = NIBSC (86/500)/CFProtocol Diagram
Linearity
The concentrations of IL-2 were measured and interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: Jurkat T cells stimulated with 10 ng/ml PMA and 150 ng/ml A23187 for 24 h (6.25%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 11,487 pg/mL in stimulated Jurkat supernatant.
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