ATP7A Recombinant Rabbit mAb (S-2289-106)

Copper-transporting ATPase 1,Copper pump 1,Menkes disease-associated protein,MC1,MNK

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6233

规格

25μl100μl1ml

数量

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

ATP7A
分子别名

Copper-transporting ATPase 1; Copper pump 1; Menkes disease-associated protein; MC1; MNK
免疫原

Synthetic Peptide
细胞定位

Cell membrane
Accession

Q04656
克隆号

S-2289-106
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

A549, HeLa, SH-SY5Y, mouse lung, C6
预测反应种属
(反应种属缩写表)

Hm
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ? ,
WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IHC-P 1:200 Hu, Ms, Rt

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IHC-P	1:200	Hu, Ms, Rt

背景介绍

ATP7A (Menkes ATPase) is a copper-transporting P-type ATPase that uses ATP hydrolysis to actively pump Cu(I) across membranes, thereby maintaining cellular copper homeostasis: under normal or low copper conditions it resides in the trans-Golgi network (TGN) and delivers copper to cuproenzymes (e.g., peptidyl-α-monooxygenase, tyrosinase, lysyl oxidase), while under high copper it traffics to the plasma membrane to efflux excess copper; the 1,500-amino-acid protein contains eight transmembrane segments forming a copper channel, an ATP-binding domain, and six N-terminal cytosolic Cu(I)-binding GMTCXXC motifs, and mutations in ATP7A cause the X-linked disorder Menkes disease, characterized by systemic copper deficiency, neurodegeneration, and early death.

免疫印迹
- WB result of ATP7A Recombinant Rabbit mAb
  Primary antibody: ATP7A Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: unboiled HepG2 whole cell lysate 20 µg
  Lane 2: unboiled A549 whole cell lysate 20 µg
  Lane 3: unboiled HeLa whole cell lysate 20 µg
  Lane 4: unboiled SH-SY5Y whole cell lysate 20 µg
  Low expression control: unboiled HepG2 whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 163 kDa
  Observed MW: 130-200 kDa
- WB result of ATP7A Recombinant Rabbit mAb
  Primary antibody: ATP7A Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: unboiled mouse lung lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 163 kDa
  Observed MW: 200 kDa
- WB result of ATP7A Recombinant Rabbit mAb
  Primary antibody: ATP7A Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: unboiled C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 163 kDa
  Observed MW: 200 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human kidney. Anti-ATP7A antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human prostate. Anti-ATP7A antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-ATP7A antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse stomach. Anti-ATP7A antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse testis. Anti-ATP7A antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat kidney. Anti-ATP7A antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat testis. Anti-ATP7A antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
- Expression of ATP7A in tumor tissue.
- Expression of ATP7A in human tissue.
- Expression of ATP7A in mouse & rat tissue.