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AKT2 Recombinant Rabbit mAb (S-3256)

RAC-beta serine/threonine-protein kinase,Protein kinase Akt-2,Protein kinase B beta (PKB beta),RAC protein kinase beta (RAC-PK-beta)

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6214
规格
数量
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产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    AKT2

  • 分子别名

    RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta (PKB beta); RAC protein kinase beta (RAC-PK-beta)

  • 细胞定位

    Nucleus, Cytoplasm

  • Accession

    P31751

  • 克隆号

    S-3256

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt, Mk

  • 阳性样本

    MEF, Jurkat, JAR, HeLa, NIH/3T3, C6, COS-7

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms, Rt, Mk
背景介绍

  • AKT2 (also known as Protein Kinase B β, PKBβ) is a 481-residue serine/threonine kinase ubiquitously expressed but enriched in insulin-responsive tissues, where it transduces signals from the PI3K pathway through dual phosphorylation at T309 by PDK1 and S474 by mTORC2 to adopt an active conformation that phosphorylates >100 substrates—key among them GSK3β, FOXO1, AS160, and mTOR—thereby orchestrating glucose uptake via GLUT4 translocation, glycogen synthesis, cell survival, proliferation, and lipid metabolism; its dysregulation through activating E17K mutations, amplifications, or loss of PTEN contributes to type 2 diabetes, metabolic syndrome, and multiple cancers, making AKT2 a pivotal therapeutic target for isoform-selective inhibitors and metabolic modulators.

  • 免疫印迹

    • WB result of Akt2 Recombinant Rabbit mAb
      Primary antibody: Akt2 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: MEF whole cell lysate 20 µg
      Lane 2: Jurkat whole cell lysate 20 µg
      Lane 3: JAR whole cell lysate 20 µg
      Lane 4: HeLa whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 56 kDa
      Observed MW: 60 kDa

    • WB result of Akt2 Recombinant Rabbit mAb
      Primary antibody: Akt2 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 56 kDa
      Observed MW: 60 kDa

    • WB result of Akt2 Recombinant Rabbit mAb
      Primary antibody: Akt2 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 56 kDa
      Observed MW: 60 kDa

    • WB result of Akt2 Recombinant Rabbit mAb
      Primary antibody: Akt2 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: COS-7 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 56 kDa
      Observed MW: 60 kDa

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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