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Phospho-FAK (Tyr397) Rabbit Polyclonal Antibody

Focal adhesion kinase 1,FADK 1,Focal adhesion kinase-related nonkinase (FRNK),Protein phosphatase 1 regulatory subunit 71 (PPP1R71),Protein-tyrosine kinase 2,p125FAK,pp125FAK,FAK,FAK1,PTK2

价格 ￥400.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6116
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产品介绍

产品介绍 FAQs 引用文献(1) 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Phospho-FAK (Tyr397)

  • 分子别名

    Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase (FRNK); Protein phosphatase 1 regulatory subunit 71 (PPP1R71); Protein-tyrosine kinase 2; p125FAK; pp125FAK; FAK; FAK1; PTK2

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoskeleton, Nucleus, Cell membrane, Cytoplasm

  • Accession

    Q05397

  • 抗体类型

    Polyclonal antibody

  • 抗体同种型

    IgG

  • 翻译后修饰类型

    磷酸化

  • 反应种属 ?

    Hu, Ms

  • 预测反应种属
    (反应种属缩写表)

    Ck, Rt, Xe

  • 纯化方式

    Immunogen Affinity

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms
    ICC 1:500 Hu
背景介绍

  • Phospho-FAK (Tyr397) is a crucial phosphorylated form of the focal adhesion kinase (FAK) protein, which plays a significant role in various cellular processes. FAK is a non-receptor protein tyrosine kinase that is involved in the regulation of cell adhesion, migration, and survival. When phosphorylated at tyrosine residue 397, FAK becomes activated and serves as a binding site for other signaling molecules, such as Src, thereby initiating downstream signaling pathways that are essential for cell motility and the formation of focal adhesions. Dysregulation of Phospho-FAK (Tyr397) has been implicated in several diseases, including cancer, where it contributes to tumor progression and metastasis by enhancing cell invasion and survival.

  • 免疫印迹

    • WB result of Phospho-FAK (Tyr397) Rabbit pAb
      Primary antibody: Phospho-FAK (Tyr397) Rabbit pAb at 1/1000 dilution
      Lane 1: untreated HeLa whole cell lysate 20 µg
      Lane 2: HeLa treated with 10 mM Pervanadate for 60 minutes whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 119 kDa
      Observed MW: 120 kDa

    • WB result of Phospho-FAK (Tyr397) Rabbit pAb
      Primary antibody: Phospho-FAK (Tyr397) Rabbit pAb at 1/1000 dilution
      Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
      Lane 2: NIH/3T3 treated with 10 mM Pervanadate for 60 minutes whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 119 kDa
      Observed MW: 120 kDa

  • 免疫细胞化学

    • ICC analysis of HeLa cells treated with Pervanadate (10mM, 60min) (top panel) and untreated HeLa cells (below panel). Anti- Phospho-FAK (Tyr397) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

检测磷酸化蛋白时，推荐有什么封闭体系？

我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高

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