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宿主来源
Goat抗体类型
Polyclonal antibody应用
FCM, ICC, IF反应种属 ?
Ms纯化方式
Immunogen Affinity浓度
2 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
| 应用 | 稀释度 |
|---|---|
| ICC | 1:1000-1:2000 |
| IF | 1:1000-1:2000 |
| FCM | 1:2000 |
流式分析
Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte, left) / Jurkat (Human T cell leukemia T lymphocyte, Right) labelling CD28 antibody (S0B0009) / (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-Mouse IgG(H+L) (Alexa Fluor® 488 Conjugate) antibody at 1/2000 (0.1 μg) dilution was used as the secondary antibody.
Negative control: THP-1
免疫细胞化学
ICC shows positive staining in Raji cells. Anti-CD19 antibody (S0B2311) was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat anti-Mouse IgG(H+L) (Alexa Fluor® 488 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
Negative control: ICC shows negative staining in Jurkat cells. Anti-CD19 antibody (S0B2311) was used at 1/500 dilution and incubated overnight at 4°C. Goat anti-Mouse IgG(H+L) (Alexa Fluor® 488 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
免疫荧光
IF shows positive staining in paraffin-embedded human spleen. Anti-CD19 antibody (S0B2311) was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat anti-Mouse IgG(H+L) (Alexa Fluor® 488 Conjugate) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human tonsil. Anti-CD19 (S0B2311) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat anti-Mouse IgG(H+L) (Alexa Fluor® 488 Conjugate) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human diffuse large B cell lymphoma. Anti-CD19 antibody (S0B2311) was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat anti-Mouse IgG(H+L) (Alexa Fluor® 488 Conjugate) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
引用文献(1)
- Modular Design of Lipopeptide‐Based Organ‐Specific Targeting (POST) Lipid Nanoparticles for Highly Efficient RNA Delivery
C Tang, Y Zhang, B Li, X Fan, Z Wang, R Su
advanced materials. 2025 Feb 09 ; 63
影响因子： 25.8
货号：S0B4017产品名称：Goat anti-Mouse IgG(H+L) (Alexa Fluor® 488 Conjugate)
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