ROR1 Recombinant Rabbit mAb (SDT-R501-148)

货号： S0B2301

Datasheet COA

价格： ￥600
规格：
25μl100μl500μl1ml
数量：

大包装询价

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产品规格

宿主来源

Rabbit
分子别名

Inactive tyrosine-protein kinase transmembrane receptor ROR1, Neurotrophic tyrosine kinase, receptor-related 1
免疫原

Synthetic Peptide & Recombinant Protein
细胞定位

Membrane
Accession

Q01973
克隆号

SDT-R501-148
抗体类型

Recombinant mAb
抗体同种型

IgG
应用

IHC-P, WB
反应种属 ?

Hu, Ms
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:1000
IHC-P	1:500

背景介绍

Tyrosine-protein kinase transmembrane receptor ROR1, also known as neurotrophic tyrosine kinase, receptor-related 1 (NTRKR1), is an enzyme that in humans is encoded by the ROR1 gene. ROR1 is a member of the receptor tyrosine kinase-like orphan receptor (ROR) family. The protein encoded by this gene is a receptor tyrosine kinase that modulates neurite growth in the central nervous system. It is a type I membrane protein and belongs to the ROR subfamily of cell surface receptors. The expression of soluble ROR1 does not differ between normal individuals and tumor patients, and the expression level in serum is low or undetectable, independent of disease progression and severity. However, intact membrane bound ROR1 is specifically expressed at high levels in various tumor tissues. ROR1 is currently under investigation for its role in the metastasis of cancer cells. ROR1 has been found in tumor cells of many different types of cancer, such as chronic lymphocytic leukemia (CLL) and other hematological malignancies. It appears that ROR1 is mainly expressed in poorly differentiated tumors with high potential for recurrence and metastasis.

免疫印迹
- WB result of ROR1 Rabbit mAb
  Primary antibody: ROR1 Rabbit mAb at 1/1000 dilution
  Lane 1: K-562 whole cell lysate 20 µg
  Lane 2: MCF7 whole cell lysate 20 µg
  Lane 3: PANC-1 whole cell lysate 20 µg
  Lane 4: MDA-MB-231 whole cell lysate 20 µg
  Lane 5: A549 whole cell lysate 20 µg
  Negative control: K-562 whole cell lysate; MCF7 whole cell lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 104 kDa
  Observed MW: 130,135 kDa
- WB result of ROR1 Rabbit mAb
  Primary antibody: ROR1 Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 104 kDa
  Observed MW: 130,135 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human ovarian carcinoma. Anti-ROR1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human gastric carcinoma. Anti-ROR1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human endometrial carcinoma. Anti-ROR1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human mantle lymphoma. Anti-ROR1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human tonsil. Anti-ROR1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.