Human folate receptor alpha Recombinant Rabbit mAb (SDT-719-20-2)

Catalog Number： S0B2275

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Product Specification

Host

Rabbit
Antigen

Human folate receptor alpha
Synonyms

FR-alpha, Adult folate-binding protein (FBP), Folate receptor 1, KB cells FBP, Ovarian tumor-associated antigen MOv18, FOLR1, FOLR
Immunogen

Synthetic Peptide
Location

Cell membrane
Accession

P15328
Clone Number

SDT-719-20-2
Antibody Type

Recombinant mAb
Isotype

IgG
Application

IHC-P, WB
Reactivity

Hu, Ms, Rat
Purification

Protein A
Concentration

0.5 mg/ml
Conjugation

Unconjugated
Physical Appearance

Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

- WB
  
  1:1000
- IHC-P
  
  1:250

Background

Folate receptor 1 (Folate receptor alpha, FOLR1) is a protein that in humans is encoded by the FOLR1 gene. The protein encoded by this gene is a member of the folate receptor (FOLR) family. Members of this family have a high affinity for folic acid and for several reduced folic acid derivatives, and mediate delivery of 5-methyltetrahydrofolate to the interior of cells. FRα, due to its high expression in some tumors, is an attractive therapeutic target for the development of novel anti-cancer agents in order to limit toxic side-effects on off-target tissues. FRa can be overexpressed by a number of epithelial-derived tumors including ovarian, breast, renal, lung, colorectal, and brain. Therefore, antibodies to FRa are being developed for use in targeted therapies, with one example being farletuzumab, in a phase III trial for ovarian cancer.

Western Blot
- WB result of Human folate receptor alpha Rabbit mAb
  Primary antibody: Human folate receptor alpha Rabbit mAb at 1/1000 dilution
  Lane 1: K562 whole cell lysate 20 µg
  Lane 2: JAR whole cell lysate 20 µg
  Lane 3: HeLa whole cell lysate 20 µg
  Negative control: K562 whole cell lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 30 kDa
  Observed MW: 38 kDa
  (This blot was developed with high sensitivity substrate)
- WB result of Human folate receptor alpha Rabbit mAb
  Primary antibody: Human folate receptor alpha Rabbit mAb at 1/1000 dilution
  Lane 1: mouse kidney lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 30 kDa
  Observed MW: 38 kDa
- WB result of Human folate receptor alpha Rabbit mAb
  Primary antibody: Human folate receptor alpha Rabbit mAb at 1/1000 dilution
  Lane 1: rat kidney lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 30 kDa
  Observed MW: 38 kDa
Immunohistochemistry
- IHC shows positive staining in paraffin-embedded human kidney. Anti-Human folate receptor alpha antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung. Anti-Human folate receptor alpha antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human placenta. Anti-Human folate receptor alpha antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human tonsil. Anti-Human folate receptor alpha antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human ovarian carcinoma. Anti-Human folate receptor alpha antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse kidney. Anti-Human folate receptor alpha antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat kidney. Anti-Human folate receptor alpha antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.