CD14 Mouse mAb,PBS Only (SDT-411-1)
CD14,150 kDa TGF-beta-1-binding protein,C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7,p180,Platelet-specific Gov antigen,Myeloid cell-specific leucine-rich glycoprotein,Monocyte differentiation antigen CD14,FMC32lipopolysaccharide (LPS)
货号： S0B2221P
- 价格： 询价
- 规格：
- 数量：
大包装询价 产品介绍 评论(0)
别名
CD14,150 kDa TGF-beta-1-binding protein,C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7,p180,Platelet-specific Gov antigen,Myeloid cell-specific leucine-rich glycoprotein,Monocyte differentiation antigen CD14,FMC32lipopolysaccharide (LPS)宿主来源
Mouse抗原名称
CD14免疫原
Synthetic Peptide细胞定位
Golgi Apparatus, Cell membrane, SecretedAccession
P08571克隆号
SDT-411-1抗体类型
Mouse mAb抗体同种型
IgG2a,k应用
IHC-P, IF反应种属 ?
Hu纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS储存条件
12 months from date of receipt, 4°C as supplied
| 应用 | 稀释度 |
|---|---|
| IHC-P | 1:1000 |
| IF | 1:500 |
CD14 (cluster of differentiation 14) is a human protein made mostly by macrophages as part of the innate immune system. It helps to detect bacteria in the body by binding lipopolysaccharide (LPS), a pathogen-associated molecular pattern (PAMP). CD14 exists in two forms, one anchored to the membrane by a glycosylphosphatidylinositol (GPI) tail (mCD14), the other a soluble form (sCD14). Soluble CD14 either appears after shedding of mCD14 (48 kDa) or is directly secreted from intracellular vesicles (56 kDa).
免疫组化
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-CD14 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CD14 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human Hodgkin's lymphoma. Anti-CD14 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-CD14 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-CD14 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD14 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-CD14 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded human tonsil. Anti-CD14 antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat anti-Mouse IgG(H+L) (Alexa Fluor® 594 Conjugate)(S0B4010) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
评论(0)