S-RMab^® p120 Catenin Recombinant Rabbit mAb (SDT-350-75)

货号： S0B2214

Datasheet

价格： ￥600
规格：
25μl100μl500μl1ml
数量：

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产品规格

宿主来源

Rabbit
抗原名称

p120 Catenin
分子别名

Catenin delta-1, Cadherin-associated Src substrate, CAS, p120 catenin, p120(ctn), p120(cas)
免疫原

Synthetic Peptide
Accession

O60716
克隆号

SDT-350-75
抗体类型

Recombinant mAb
应用

IHC-P, ICC, WB, FC(Intra)
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:1000
ICC	1:1000
IHC-P	1:500-1:1000
FC(Intra)	1:500

背景介绍

Catenin δ-1 (P120 Catenin) belongs to the β-serial protein family .p-120 catenin (along with α, β and γ catenins) connects the transmembrane protein E-cadherin to the actin cytoskeleton in the cell cytoplasm. It expresses in various normal epithelial tissues (including thyroid, breast, stomach, duodenal, liver, pancreas, colon, kidney, prostate, testicles), various non -epithelial tissues (including myocardial cells, neurons, gel cells To. p-120 regulates the transformation of calcium adhesion protein on the surface of the cells, thereby determining the E-calcium adhesive protein levels used in the cells, and then plays an important role in the adhesion of the cells. Many studies have shown that p-120 irregularly expression or lack of disobedience in a variety of cancer cells indicates that p-120 play a role in cancer-suppressing genes.

免疫印迹
- WB result of p120 Catenin Rabbit mAb Primary antibody: p120 Catenin Rabbit mAb at 1/1000 dilution Lane 1: HL-60 whole cell lysate 20 µg Lane 2: HT-29 whole cell lysate 20 µg Lane 3: HeLa whole cell lysate 20 µg Lane 4: A431 whole cell lysate 20 µg Negative control: HL-60 whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 108 kDa Observed MW: 70~105 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HL-60 (Human acute promyelocytic leukemia promyeloblast, left) / HT29 (Human colorectal adenocarcinoma epithelial cell, Right) cells labelling E-Cadherin antibody at 1/500 dilution (0.1 μg) / (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Negative control: HL-60
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-p120 Catenin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human stomach. Anti-p120 Catenin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-p120 Catenin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-p120 Catenin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse stomach. Anti-p120 Catenin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat liver. Anti-p120 Catenin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat esophagus. Anti-p120 Catenin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human invasive lobular breast cancer. Anti-p120 Catenin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HT-29 cells. Anti-p120 Catenin antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
- Negative control: ICC shows negative staining in HL-60 cells. Anti-p120 Catenin antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).