β-Catenin Recombinant Rabbit mAb,PBS Only (SDT-336-23)

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货号： S0B2203P

Datasheet COA

价格：询价
规格：
1mg
数量：

大包装询价

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产品规格

宿主来源

Rabbit
抗原名称

β-Catenin
分子别名

Catenin beta-1, Beta-catenin，CTNNB1
免疫原

Synthetic Peptide
细胞定位

Cell membrane, Cytoplasm
Accession

P35222
克隆号

SDT-336-23
抗体类型

Recombinant mAb
应用

IHC-P, WB, IP, IF
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

1 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS
储存条件

12 months from date of receipt, 4°C as supplied

稀释度

应用	稀释度
WB	1:1000
IHC-P	1:500
IP	1:50
IF	1:500

背景介绍

β-catenin is a multifunction protein with a central role in physiological homeostasis. Its abnormal expression leads to various diseases including cancer. In normal physiology, β-catenin either maintains integrity of epithelial tissues or controls transcription of various genes on extracellular instigations. In epithelial tissues, β-catenin functions as a component of the cadherin protein complex and regulates epithelial cell growth and intracellular adhesion [PMID: 23490077].

免疫印迹
- WB result of β-Catenin Rabbit mAb
  Primary antibody: β-Catenin Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: HepG2 whole cell lysate 20 µg
  Lane 3: A431 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 85kDa
  Observed MW: 92kDa
- WB result of β-Catenin Rabbit mAb
  Primary antibody: β-Catenin Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 85kDa
  Observed MW: 92kDa
- WB result of β-Catenin Rabbit mAb
  Primary antibody: β-Catenin Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 85kDa
  Observed MW: 92kDa
免疫沉淀
- β-Catenin Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating β-Catenin in 0.4 mg A431 whole cell lysate.
  Western blot was performed on the immunoprecipitate using β-Catenin Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: A431 whole cell lysate 20 µg (Input)
  Lane 2: β-Catenin Rabbit mAb IP in A431 whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in A431 whole cell lysate
  Predicted MW: 85 kDa
  Observed MW: 92 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human colon. Anti-β-Catenin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-β-Catenin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-β-Catenin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-β-Catenin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-β-Catenin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-β-Catenin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human prostatic carcinoma. Anti-β-Catenin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse liver. Anti-β-Catenin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat kidney. Anti-β-Catenin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
- IF shows positive staining in paraffin-embedded mouse liver. Anti-β-Catenin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded rat liver. Anti-β-Catenin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.