GPA33 Recombinant Rabbit mAb (SDT-248-76)

货号： S0B2168

Datasheet

价格： ￥600
规格：
25μl100 μl500μl1ml
数量：

大包装询价

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产品规格

宿主来源

Rabbit
抗原名称

GPA33
分子别名

Cell surface A33 antigen, Glycoprotein A33
免疫原

Synthetic Peptide
细胞定位

Membrane
Accession

Q99795
克隆号

SDT-248-76
抗体类型

Recombinant mAb
应用

IHC-P, WB
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.25 mg/ml
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:500
IHC-P	1:2000

背景介绍

The A33 cell-surface differentiation antigen is a glycoprotein that belongs to the immunoglobulin superfamily [PMID: 9012807]. This antigen is expressed in >95% of primary and metastatic colon cancer cells, but is absent in most normal and tumor cell types [PMID: 9245713, PMID: 16000578]. Although the exact function of this glycoprotein is unknown, antibodies developed against the A33 antigen bind to tumor cells and exhibit prolonged retention in the tumor tissue [PMID: 8656247].

免疫印迹
- WB result of GPA33 Rabbit mAb
  Primary antibody: GPA33 Rabbit mAb at 1/500 dilution
  Lane 1: HT-29 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 36 kDa
  Observed MW: 50 kDa
  Exposure time: 180 s(This blot was developed with high sensitivity substrate)
免疫组化
- IHC shows positive staining in paraffin-embedded human colon. Anti-GPA33 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-GPA33 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-GPA33 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-GPA33 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human breast cancer. Anti-GPA33 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-GPA33 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.