S-RMab^® p53 Recombinant Rabbit mAb (SDT-R118)

货号： S0B2146

Datasheet COA

价格： ￥600
规格：
25μl100μl500μl1ml
数量：

大包装询价

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产品规格

宿主来源

Rabbit
抗原名称

p53
分子别名

Tumor suppressor p53, TP53
免疫原

N/A
细胞定位

Nucleus
Accession

P04637
克隆号

SDT-R118
抗体类型

Rabbit mAb
应用

IHC-P, ICC, WB, IP, IF
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.25 mg/ml
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
IHC-P	1:1000
WB	1:500
IP	1:25
ICC	1:250
IF	1:500

背景介绍

P53 is a gene that encodes for the p53 tumor suppressor protein, commonly referred to as the “Guardian of the Genome” [PMID: 1614522]The p53 protein is a transcription factor that controls the output of many biological processes according to the type of cellular stress signal input. Stress signals known to activate p53 include oncogene activation, DNA damage, and replication stress [PMID: 28886379]. In response to these stresses, p53 undergoes post-translational modifications, promotes the transcription of genes involved in specific cell responses according to the stress type thereby controlling the cell’s fate [PMID: 15510160].When the p53 protein is mutated or aggregated, it loses its function, resulting in tumor progression and growth [PMID: 29789497].

免疫印迹
- WB result of P53 Rabbit mAb
  Primary antibody: P53 Rabbit mAb at 1/500 dilution
  Lane 1: A431 whole cell lysate 20 µg
  Lane 2: MCF7 whole cell lysate 20 µg
  Lane 3: HT-29 whole cell lysate 20 µg
  Lane 4: T-47D whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 53 kDa
  Observed MW: 53 kDa
免疫沉淀
- p53 Rabbit mAb at 1/25 dilution (1 µg) immunoprecipitating p53 in 0.4 mg A431 whole cell lysate.
  Western blot was performed on the immunoprecipitate using p53 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: A431 whole cell lysate 20 µg (Input)
  Lane 2: p53 Rabbit mAb IP in A431 whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in A431 whole cell lysate
  Predicted MW: 53 kDa
  Observed MW: 53 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-p53 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-p53 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-p53 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-p53 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human NK/T-cell lymphoma. Anti-p53 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human chromophobe renal cell carcinoma. Anti-p53 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in A431 cells. Anti-p53 antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).
免疫荧光
- IF shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-p53 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.