S-RMab^® Keratin 18 Recombinant Rabbit mAb (SDT-012-7)

货号： S0B2131

Datasheet

价格： ￥600
规格：
25μl100μl500μl1ml
数量：

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产品规格

宿主来源

Rabbit
抗原名称

Keratin 18
分子别名

Cytokeratin-18, CK-18
免疫原

Synthetic Peptide
细胞定位

Cytoplasm
Accession

P05783
克隆号

SDT-012-7
抗体类型

Rabbit mAb
应用

IHC-P, ICC, WB, IP, FC(Intra)
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Cz, Or, Pr, Gor
纯化方式

Protein A
浓度

0.05 mg/ml
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:1000
IHC-P	1:2000
ICC	1:50
FC(Intra)	1:50
IP	1:25

背景介绍

Cytokeratins are proteins of cytoskeletal intermediate filaments, and their main function is to enable cells to withstand mechanical stress. In humans, 20 different cytokeratin isotypes have been identified. Cytokeratins 8, 18, 19, and 20 have been associated with bladder. Cytokeratin 18 (KRT18, also called K18), found in epithelial cells, is released from hepatocytes upon death.

免疫印迹
- WB result of Keratin 18 Rabbit mAb
  Primary antibody: Keratin 18 Rabbit mAb at 1/1000 dilution
  Lane 1: MCF7 whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 46 kDa
  Observed MW: 42 kDa
  Exposure time: 6s
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) labelling Keratin 18 antibody at 1/50 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
- Keratin 18 Rabbit mAb at 1/25 dilution (0.2 µg) immunoprecipitating Keratin 18 in 0.2 mg HeLa whole cell lysate.
  Western blot was performed on the immunoprecipitate using Keratin 18 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: HeLa whole cell lysate 5 µg (Input)
  Lane 2: Keratin 18 Rabbit mAb IP in HeLa whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
  Predicted MW: 46 kDa
  Observed MW: 42 kDa
  Exposure time: 30 s
免疫组化
- IHC shows positive staining in paraffin-embedded human liver. Anti-Keratin 18 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-Keratin 18 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-Keratin 18 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human ovarian carcinoma. Anti-Keratin 18 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative tissue: IHC shows negative staining in paraffin-embedded human lung squamous cell carcinoma. Anti-Keratin 18 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative tissue: IHC shows negative staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Keratin 18 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse liver. Anti-Keratin 18 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat liver. Anti-Keratin 18 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in MCF7 cells. Anti-Keratin 18 antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).