S-RMab^® CK-Pan Recombinant Rabbit mAb (SDT-P001)

Catalog Number： S0B2118

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Product Specification

Host

Rabbit
Antigen

CK-Pan
Synonyms

Pan Cytokeratin, Pan CK
Immunogen

N/A
Location

Cytoplasm
Clone Number

SDT-P001
Antibody Type

Rabbit mAb
Application

IHC-P, WB, IF
Reactivity

Hu
Purification

Protein A
Concentration

Lot specific* (generally 0.3 to 0.5 mg/ml)*
Physical Appearance

Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

- IHC-P
  
  1:500-1:2000
- WB
  
  1:1000
- IF
  
  1:500

Background

CK (Pan) mainly marks keratinized epithelium, stratified squamous epithelium, stratified epithelium, proliferative keratinocytes, and monolayer epithelium, and is used in squamous cell carcinoma (including Spindle variant), various adenocarcinoma (including adrenal carcinoma and hepatocellular carcinoma), transitional cell carcinoma, small cell carcinoma, malignant mesothelioma, germ cell tumor (excluding spermatogonium Tumor, partial synovial sarcoma, and leiomyosarcoma can be positively labeled. Pathology is mainly applied to the differential diagnosis of epithelial tumors and non epithelial tumors.

Western Blot
- WB result of CK-Pan Rabbit mAb
  Primary antibody: CK-Pan Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 40~70 kDa
  Observed MW: 40~55 kDa
Immunohistochemistry
- IHC shows positive staining in paraffin-embedded human colon. Anti-CK-Pan antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-CK-Pan antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-CK-Pan antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-CK-Pan antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human pancreatic carcinoma. Anti-CK-Pan antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
- IF shows positive staining in paraffin-embedded human breast cancer. Anti-CK-Pan antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-CK-Pan antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CK-Pan antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.