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S-RMab® CK-Pan Recombinant Rabbit mAb (SDT-P001)

CHKA,胆碱激酶α,CHK,CKI,Choline kinase alpha,CK,CHETK-alpha,Ethanolamine kinase,EK

货号: S0B2118

Datasheet COA
  • 价格: ¥600
  • 规格:
  • 数量:
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产品介绍 评论(0)

产品规格
  • 别名

    CHKA,胆碱激酶α,CHK,CKI,Choline kinase alpha,CK,CHETK-alpha,Ethanolamine kinase,EK
  • 宿主来源

    Rabbit
  • 抗原名称

    CK-Pan
  • 分子别名

    Pan Cytokeratin, Pan CK
  • 免疫原

    N/A
  • 细胞定位

    Cytoplasm
  • 克隆号

    SDT-P001
  • 抗体类型

    Rabbit mAb
  • 应用

    IHC-P, WB, IF
  • 反应种属 ?

    Hu
  • 纯化方式

    Protein A
  • 浓度

    Lot specific* (generally 0.3 to 0.5 mg/ml)*
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied
稀释度
应用 稀释度
IHC-P 1:500-1:2000
WB 1:1000
IF 1:500
背景介绍
  • CK (Pan) mainly marks keratinized epithelium, stratified squamous epithelium, stratified epithelium, proliferative keratinocytes, and monolayer epithelium, and is used in squamous cell carcinoma (including Spindle variant), various adenocarcinoma (including adrenal carcinoma and hepatocellular carcinoma), transitional cell carcinoma, small cell carcinoma, malignant mesothelioma, germ cell tumor (excluding spermatogonium Tumor, partial synovial sarcoma, and leiomyosarcoma can be positively labeled. Pathology is mainly applied to the differential diagnosis of epithelial tumors and non epithelial tumors.

  • 免疫印迹

    • WB result of CK-Pan Rabbit mAb                            

      Primary antibody: CK-Pan Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 40~70 kDa
      Observed MW: 40~55 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human colon. Anti-CK-Pan antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-CK-Pan antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-CK-Pan antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-CK-Pan antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human pancreatic carcinoma. Anti-CK-Pan antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫荧光

    • IF shows positive staining in paraffin-embedded human breast cancer. Anti-CK-Pan antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • IF shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-CK-Pan antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • IF shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CK-Pan antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

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