大包装询价 产品介绍 评论(0)
别名
CHKA,胆碱激酶α,CHK,CKI,Choline kinase alpha,CK,CHETK-alpha,Ethanolamine kinase,EK宿主来源
Rabbit抗原名称
CK-Pan分子别名
Pan Cytokeratin, Pan CK免疫原
N/A细胞定位
Cytoplasm克隆号
SDT-P001抗体类型
Rabbit mAb应用
IHC-P, WB, IF反应种属 ?
Hu纯化方式
Protein A浓度
Lot specific* (generally 0.3 to 0.5 mg/ml)*性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| IHC-P | 1:500-1:2000 |
| WB | 1:1000 |
| IF | 1:500 |
CK (Pan) mainly marks keratinized epithelium, stratified squamous epithelium, stratified epithelium, proliferative keratinocytes, and monolayer epithelium, and is used in squamous cell carcinoma (including Spindle variant), various adenocarcinoma (including adrenal carcinoma and hepatocellular carcinoma), transitional cell carcinoma, small cell carcinoma, malignant mesothelioma, germ cell tumor (excluding spermatogonium Tumor, partial synovial sarcoma, and leiomyosarcoma can be positively labeled. Pathology is mainly applied to the differential diagnosis of epithelial tumors and non epithelial tumors.
免疫印迹
WB result of CK-Pan Rabbit mAb
Primary antibody: CK-Pan Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40~70 kDa
Observed MW: 40~55 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-CK-Pan antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-CK-Pan antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-CK-Pan antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-CK-Pan antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic carcinoma. Anti-CK-Pan antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded human breast cancer. Anti-CK-Pan antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-CK-Pan antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CK-Pan antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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