S-RMab^® CD45 Recombinant Rabbit mAb (SDT-R035)

Catalog Number： S0B2087

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Product Specification

Host

Rabbit
Antigen

CD45
Synonyms

Leukocyte common antigen (L-CA)
Immunogen

N/A
Location

Cell membrane
Accession

P08575
Clone Number

SDT-R035
Antibody Type

Rabbit mAb
Application

IHC-P, ICC, WB
Reactivity

Hu
Purification

N/A
Concentration

0.25 mg/ml
Conjugation

Unconjugated
Physical Appearance

Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

- WB
  
  1:2500
- IHC-P
  
  1:250-1:1000
- ICC
  
  1:250

Background

CD45 is a type I transmembrane protein that is present in various isoforms on all differentiated hematopoietic cells (except erythrocytes and plasma cells). CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes via its extracellular domain (a form of co-stimulation), or by activating various Src family kinases required for the antigen receptor signaling via its cytoplasmic domain. CD45 also suppresses JAK kinases, and so functions as a negative regulator of cytokine receptor signaling.

Western Blot
- WB result of CD45 Rabbit mAb
  Primary antibody: CD45 Rabbit mAb at 1/2500 dilution
  Lane 1: MCF7 whole cell lysate 20 µg
  Lane 2: Raji whole cell lysate 20 µg
  Lane 3: Jurkat whole cell lysate 20 µg
  Negative control: MCF7 whole cell lysate
  Low expression control: Raji whole cell lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 180~240 kDa
  Observed MW: 240~260 kDa
Immunohistochemistry
- IHC shows positive staining in paraffin-embedded human tonsil.
  Anti-CD45 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human spleen.
  Anti-CD45 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon.
  Anti-CD45 antibody was used at 1/250 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon.
  Anti-CD45 antibody was used at 1/250 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cervix cancer.
  Anti-CD45 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protoco
- IHC shows positive staining in paraffin-embedded human diffuse large B-cell lymphoma.
  Anti-CD45 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control:IHC shows negative staining in paraffin-embeddedhuman skeletal muscle.
  Anti-CD45 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
- ICC shows positive staining in Jurkat cells. Anti-CD45 antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).
- Negative control:ICC shows negative staining in MCF7 cells. Anti-CD45 antibody was used at 1/250 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).