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S-RMab® c-Met Recombinant Rabbit mAb (SDT-009-7H0L0)

Catalog Number: S0B2052

Datasheet
  • Price: $45
  • Size:
  • Quantity:
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Product Specification
  • Host

    Rabbit
  • Antigen

    c-Met
  • Synonyms

    HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor (SF receptor), Tyrosine-protein kinase Met
  • Immunogen

    Synthetic Peptide
  • Location

    Membrane, Cytoplasm
  • Accession

    P08581
  • Clone Number

    SDT-009-7H0L0
  • Antibody Type

    Rabbit mAb
  • Isotype

    IgG
  • Application

    IHC-P, WB
  • Reactivity

    Hu
  • Purification

    Protein A
  • Concentration

    2mg/ml
  • Molecular Weight

    140kDa,170kDa
  • Conjugation

    Unconjugated
  • Physical Appearance

    Liquid
  • Storage Buffer

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
  • Stability & Storage

    12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution
    • IHC-P

      1:300
    • WB

      1:40000
Background
  • c-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGFR), is a protein that in humans is encoded by the MET gene. The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. MET is a single pass tyrosine kinase receptor essential for embryonic development, organogenesis and wound healing. Hepatocyte growth factor/Scatter Factor (HGF/SF) and its splicing isoform (NK1, NK2) are the only known ligands of the MET receptor. MET is normally expressed by cells of epithelial origin, while expression of HGF/SF is restricted to cells of mesenchymal origin. When HGF/SF binds its cognate receptor MET it induces its dimerization through a not yet completely understood mechanism leading to its activation. Abnormal MET activation in cancer correlates with poor prognosis, where aberrantly active MET triggers tumor growth, formation of new blood vessels (angiogenesis) that supply the tumor with nutrients, and cancer spread to other organs (metastasis). MET is deregulated in many types of human malignancies, including cancers of kidney, liver, stomach, breast, and brain. Normally, only stem cells and progenitor cells express MET, which allows these cells to grow invasively in order to generate new tissues in an embryo or regenerate damaged tissues in an adult. However, cancer stem cells are thought to hijack the ability of normal stem cells to express MET, and thus become the cause of cancer persistence and spread to other sites in the body. Both the overexpression of Met/HGFR, as well as its autocrine activation by co-expression of its hepatocyte growth factor ligand, have been implicated in oncogenesis.

  • Western Blot

    • item.picture[0].fileOrgName

      WB result of c-Met Rabbit mAb                    

      Primary antibody: c-Met Rabbit mAb at 1/40000 dilution
      Lane 1: Hela lysate 20 µg
      Lane 2: HT-29 20 µg

      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 155 kDa
      Observed MW: 155 kDa

  • Immunohistochemistry

    • item.picture[0].fileOrgName

      IHC shows positive staining in paraffin-embedded human colon cancer. Anti-c-Met antibody was used at 1/300 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • item.picture[0].fileOrgName

      IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-c-Met antibody was used at 1/300 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • item.picture[0].fileOrgName

      IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-c-Met antibody was used at 1/300 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • item.picture[0].fileOrgName

      IHC shows positive staining in paraffin-embedded human pancreatic cancer.

      Anti-c-Met antibody was used at 1/300 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • item.picture[0].fileOrgName

      IHC shows weak staining in paraffin-embedded human ovarian cancer.

      Anti-c-Met antibody was used at 1/300 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

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