S-RMab^® c-Met Recombinant Rabbit mAb (SDT-009-7H0L0)

Product Introduction

• Host

  Rabbit

• Antigen

  c-Met

• Synonyms

  HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor (SF receptor), Tyrosine-protein kinase Met

• Immunogen

  Synthetic Peptide

• Location

  Membrane, Cytoplasm

• Accession

  P08581

• Clone Number

  SDT-009-7H0L0

• Antibody Type

  Rabbit mAb

• Isotype

  IgG

• Application

  IHC-P, WB

• Reactivity

  Hu

• Purification

  Protein A

• Concentration

  2mg/ml

• Molecular Weight

  140kDa,170kDa

• Conjugation

  Unconjugated

• Physical Appearance

  Liquid

• Storage Buffer

  PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

• Stability & Storage

  12 months from date of receipt / reconstitution, -20 °C as supplied

• • IHC-P

    1:300

  • WB

    1:40000

• c-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGFR), is a protein that in humans is encoded by the MET gene. The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. MET is a single pass tyrosine kinase receptor essential for embryonic development, organogenesis and wound healing. Hepatocyte growth factor/Scatter Factor (HGF/SF) and its splicing isoform (NK1, NK2) are the only known ligands of the MET receptor. MET is normally expressed by cells of epithelial origin, while expression of HGF/SF is restricted to cells of mesenchymal origin. When HGF/SF binds its cognate receptor MET it induces its dimerization through a not yet completely understood mechanism leading to its activation. Abnormal MET activation in cancer correlates with poor prognosis, where aberrantly active MET triggers tumor growth, formation of new blood vessels (angiogenesis) that supply the tumor with nutrients, and cancer spread to other organs (metastasis). MET is deregulated in many types of human malignancies, including cancers of kidney, liver, stomach, breast, and brain. Normally, only stem cells and progenitor cells express MET, which allows these cells to grow invasively in order to generate new tissues in an embryo or regenerate damaged tissues in an adult. However, cancer stem cells are thought to hijack the ability of normal stem cells to express MET, and thus become the cause of cancer persistence and spread to other sites in the body. Both the overexpression of Met/HGFR, as well as its autocrine activation by co-expression of its hepatocyte growth factor ligand, have been implicated in oncogenesis.

• Western Blot

  • 

    WB result of c-Met Rabbit mAb                    

    Primary antibody: c-Met Rabbit mAb at 1/40000 dilution
    Lane 1: Hela lysate 20 µg
    Lane 2: HT-29 20 µg

    Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
    Predicted MW: 155 kDa
    Observed MW: 155 kDa
    Exposure time: 1s

• Immunohistochemistry

  • 

    IHC shows positive staining in paraffin-embedded human colon cancer. Anti-c-Met antibody was used at 1/300 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    

  • 

    IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-c-Met antibody was used at 1/300 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    

  • 

    IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-c-Met antibody was used at 1/300 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
    

  • 

    IHC shows positive staining in paraffin-embedded human pancreatic cancer.

    Anti-c-Met antibody was used at 1/300 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Counterstained with hematoxylin.

    Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 

    IHC shows weak staining in paraffin-embedded human ovarian cancer.

    Anti-c-Met antibody was used at 1/300 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Counterstained with hematoxylin.

    Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.