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宿主来源
Rabbit抗原名称
G4S Linker免疫原
Synthetic Peptide克隆号
S-711-23抗体类型
Recombinant mAb抗体同种型
IgG应用
FCM纯化方式
Protein A浓度
20μg/ml标记
Pacific Blue性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 5 μl per million cells in 100μl volume | Species independent |
G4S Linker is a commonly used flexible amino acid linker that plays a significant role in antibody expression and design. The sequence of this linker is (Gly-Gly-Gly-Gly-Ser)n, where "n" represents the number of repetitions. By altering the value of "n," the length of the GS amino acid chain can be optimized to achieve appropriate separation of antibody or protein domain functions or to maintain necessary inter-domain interactions. G4S Linker is typically used in single-chain variable fragments (scFv) and chimeric antigen receptors (CARs) to connect the variable heavy (VH) and variable light (VL) domains for the recognition of target antigens. The length of this linker is crucial for controlling the stability of scFv and the non-covalent association between the VH and VL domains. In CAR structures, the G4S Linker is located in the extracellular domain of the scFv, helping to maintain the structure and function of the CAR. Additionally, G4S Linker is used to detect CAR cells designed with G4S Linker, which offers high specificity and sensitivity.
流式分析
Flow cytometric analysis ScFv based CAR containing a G4S linker transfected 293T (Human embryonic kidney epithelial cell) (Right panel) or 293T (Left panel) was stained with SDT G4S Linker Recombinant Rabbit mAb (Pacific blue Conjugate) at 5 μl/test . Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
示意图
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