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Ki67 Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (SDT-R459)

Proliferation marker protein Ki-67,Antigen identified by monoclonal antibody Ki-67,Antigen KI-67,MKI67

价格 ￥1,056.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B1578
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Ki67

  • 分子别名

    Proliferation marker protein Ki-67; Antigen identified by monoclonal antibody Ki-67; Antigen KI-67; MKI67

  • 细胞定位

    Nucleus

  • Accession

    P46013

  • 克隆号

    SDT-R459

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    2 mg/ml

  • 标记

    Alexa Fluor® 488

  • 性状

    Liquid

  • 缓冲体系

    PBS, 1% BSA, 0.3% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

  • 应用

    ICC ? ,
    IF ?

  • 稀释度

    应用 稀释度
    ICC 1:200
    IF 1:200
背景介绍

  • Antigen Ki-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore, it is associated with ribosomal RNA transcription. Ki-67 was present in the nuclei of cells in the G1, S, and G2 phases of the cell division cycle as well as in mitosis. Quiescent or resting cells in the G0 phase did not express the Ki-67 antigen. Ki-67 protein was used as diagnostic tools in different types of neoplasms because it was present in all proliferating cells (normal and tumor cells) and was an excellent operational marker to determine the growth fraction of a given cell population.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti- Ki67 (Alexa Fluor® 488 Conjugate) antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

    • ICC shows positive staining in NIH/3T3 cells. Anti- Ki67 (Alexa Fluor® 488 Conjugate) antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

    • ICC shows positive staining in C6 cells. Anti- Ki67 (Alexa Fluor® 488 Conjugate) antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

  • 免疫荧光

    • IF shows positive staining in paraffin-embedded human gastric cancer. Anti- Ki67 (Alexa Fluor® 488 Conjugate) antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • IF shows positive staining in paraffin-embedded human tonsil. Anti- Ki67 (Alexa Fluor® 488 Conjugate) antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • IF shows positive staining in paraffin-embedded mouse testis. Anti- Ki67 (Alexa Fluor® 488 Conjugate) antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • IF shows positive staining in paraffin-embedded rat testis. Anti- Ki67 (Alexa Fluor® 488 Conjugate) antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

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