产品中心 蛋白翻译后修饰(PTM)抗体 位点特异性修饰抗体
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宿主来源
Rabbit抗原名称
Histone H4 (acetyl K20)分子别名
H4K20ac免疫原
Synthetic Peptide细胞定位
NucleusAccession
P62805克隆号
S-846-491抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
乙酰化反应种属 ?
Hu, Ms纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ChIP
ICC ?
WB
稀释度
应用 稀释度 推荐种属 Dot Blot 1:1000 WB 1:1000 Hu, Ms ICC 1:500 Hu ChIP 1:20-1:50 Hu
Histone H4 (acetyl K20), or H4K20ac, is a post-translational modification that is generally associated with gene repression in human cells. Contrary to most histone acetylation events that are linked to gene activation, H4K20ac is enriched around the transcription start sites (TSSs) of minimally expressed genes and within the gene body of expressed genes. This unique acetylation mark shows little correlation with known histone modifications, including histone H3 methylations. Transcriptional activators are mostly excluded from genes enriched with H4K20ac, while the transcription repressor NRSF/REST co-localizes with this mark, suggesting that H4K20ac plays a distinct role in the epigenetic regulation of gene expression.
免疫印迹
WB result of Histone H4 (acetyl K20) Recombinant Rabbit mAb
Primary antibody: Histone H4 (acetyl K20) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 11 kDa
Observed MW: 17 kDa
This blot was developed with high sensitivity substrateWB result of Histone H4 (acetyl K20) Recombinant Rabbit mAb
Primary antibody: Histone H4 (acetyl K20) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 11 kDa
Observed MW: 17 kDa
This blot was developed with high sensitivity substrate
斑点杂交
Dot blot result of Histone H4 (acetyl K20) Recombinant Rabbit mAb
Lane 1: Histone H4K20 acetyl peptide
Lane 2: Histone H4 unmodified peptide
Primary antibody: Histone H4 (acetyl K20) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫细胞化学
ICC analysis of HeLa cells treated with Trichostatin A (1 μM, 18 hr) (top panel) and HeLa cells untreated with Trichostatin A (below panel). Anti- Histone H4 (acetyl K20) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ChIP
Chromatin immunoprecipitation (ChIP) was performed on HeLa + Sodium butyrate (30mM, 4hr) cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Histone H4 (acetyl K20) Recombinant Rabbit mAb (S-846-491) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
qPCR showed the enrichment of CAMK2N1, FAM43B and MUL1 in Histone H4
(acetyl K20) Recombinant Rabbit mAb (S-846-491)-immunoprecipitated sample.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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