Histone H3 (acetyl K36) Recombinant Rabbit mAb (S-1732-53)

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1224

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Histone H3 (acetyl K36)
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

P68431
克隆号

S-1732-53
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

乙酰化
反应种属 ?

Hu, Ms
阳性样本

HeLa, NIH/3T3
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ChIP

WB
稀释度

应用稀释度推荐种属

Dot Blot 1:1000
WB 1:1000 Hu, Ms
ChIP 1:20-1:50 Hu

应用	稀释度	推荐种属
Dot Blot	1:1000
WB	1:1000	Hu, Ms
ChIP	1:20-1:50	Hu

背景介绍

Histone H3 acetylation at lysine 36 (H3K36ac) is a post-translational modification that plays a crucial role in chromatin regulation. It is predominantly found at the promoters of actively transcribed genes and is associated with the regulation of transcription, as it can influence chromatin structure, promote the recruitment of transcriptional activators, and modulate the transition between active and repressive chromatin states. This modification is also implicated in processes such as splicing, nucleosome turnover, DNA repair, and the establishment of DNA methylation patterns, highlighting its importance in maintaining genomic stability and regulating gene expression.

免疫印迹
- WB result of Histone H3 (acetyl K36) Recombinant Rabbit mAb
  Primary antibody: Histone H3 (acetyl K36) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated HeLa whole cell lysate 20 µg
  Lane 2: HeLa treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 15 kDa
  Observed MW: 17 kDa
- WB result of Histone H3 (acetyl K36) Recombinant Rabbit mAb
  Primary antibody: Histone H3 (acetyl K36) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
  Lane 2: NIH/3T3 treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 15 kDa
  Observed MW: 17 kDa
斑点杂交
- Dot blot result of Histone H3 (acetyl K36) Recombinant Rabbit mAb
  Lane 1: Histone H3K36 acetyl peptide
  Lane 2: Histone H3K36 unmodified peptide
  Primary antibody: Histone H3 (acetyl K36) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
ChIP
- Chromatin immunoprecipitation (ChIP) was performed on HeLa+TSA(500ng/ml,4h) cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Histone H3 (acetyl K36) Recombinant Rabbit mAb (S-1732-53) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation. Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
  qPCR (%input: immunoprecipitated DNA/input DNA)
  showed the enrichment of RPL30, GAPDH, MYOD1,
  AFM, SAT-α and SAT-2 in Histone H3 (acetyl K36) Recombinant Rabbit mAb
  (S-1732-53)-immunoprecipitated sample.