所有图片（1/3）

Phospho-NF-κB p65 (S529) Recombinant Rabbit mAb (S-1125-59)

Transcription factor p65,Nuclear factor NF-kappa-B p65 subunit,Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3,RELA,NFKB3

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1210

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

Phospho-NF-κB p65 (S529)
分子别名

Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; RELA; NFKB3
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
克隆号

S-1125-59
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Hu
阳性样本

HeLa
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ChIP

WB
稀释度

应用稀释度推荐种属

Dot Blot 1:1000
WB 1:1000 Hu
ChIP 1:20-1:50 Hu

背景介绍

Phosphorylation of NF-κB p65 at Ser529 is a post-translational modification that plays a significant role in the regulation of NF-κB activity. This modification is catalyzed by casein kinase II (CK2) upon stimulation by cytokines such as interleukin-1β (IL-1β) or tumor necrosis factor α (TNFα), leading to increased p65 transactivation potential in a gene-specific manner. Phosphorylation at S529 enhances the transcriptional activity of NF-κB, which is crucial for regulating various cellular processes including inflammation, immune responses, and cell survival. Dysregulation of NF-κB signaling, including phosphorylation at S529, has been implicated in various diseases, particularly in the context of chronic inflammation and aging. Phosphorylation of p65 at S529 has also been reported to promote tumorigenesis in various tissues, including the bone, breast, and head and neck, suggesting a significant role of NF-κB phosphorylation in different types of cancers.

免疫印迹
- WB result of Phospho-NF-κB p65 (S529) Recombinant Rabbit mAb
  Primary antibody: Phospho-NF-κB p65 (S529) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated HeLa whole cell lysate 20 µg
  Lane 2: HeLa treated with 20 ng/ml TNF-alpha for 5 miniutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 60 kDa
  Observed MW: 65 kDa
斑点杂交
- Dot blot result of Phospho-NF-κB p65 (S529) Recombinant Rabbit mAb
  Lane 1: NF-κB p65 (S529) phospho peptide
  Lane 2: NF-κB p65 unmodified peptide
  Primary antibody: Phospho-NF-κB p65 (S529) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
ChIP
- Chromatin immunoprecipitation (ChIP) was performed on HeLa+hTNF-α (20 ng/ml, 5 min) cells cross-linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication.
  Parallel reactions used Phospho NF-κB p65 (S529) Recombinant Rabbit mAb (S-1125-59) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation. Post - immunoprecipitation, both samples were washed, eluted, and cross-links reversed. Purified DNA was analyzed by qPCR.
  qPCR showed the enrichment of IL-8 and SAT-α in Phospho-NF-κB
  p65 (S529) Recombinant Rabbit mAb (S-1125-59)-immunoprecipitated sample.