Phospho-PAK2 (Ser20) Recombinant Rabbit mAb (S-507-231)

Serine/threonine-protein kinase PAK 2,Gamma-PAK,PAK65,S6/H4 kinase,p21-activated kinase 2 (PAK-2),p58

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1067

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Phospho-PAK2 (Ser20)
分子别名

Serine/threonine-protein kinase PAK 2; Gamma-PAK; PAK65; S6/H4 kinase; p21-activated kinase 2 (PAK-2); p58
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

Q13177
克隆号

S-507-231
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Hu, Ms, Rt
阳性样本

HeLa treated with Calyculin A, NIH/3T3 treated with Calyculin A, C6 treated with Calyculin A
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IP 1:50 Ms
ICC 1:500 Ms

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IP	1:50	Ms
ICC	1:500	Ms

背景介绍

Phospho-PAK2 (Ser20) refers to the p21-activated kinase 2 (PAK2) protein that has been phosphorylated at the serine residue number 20. PAK2 is a serine/threonine kinase involved in various signaling pathways, including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis, and proliferation. It acts as a downstream effector of the small GTPases CDC42 and RAC1. When active, CDC42 and RAC1 bind to PAK2, causing a conformational change and subsequent autophosphorylation on several serine and/or threonine residues, including Ser20. Phosphorylation at Ser20 is significant because it can affect the activation state of PAK2 and its ability to interact with other proteins. For instance, phosphorylation of PAK2 at Ser20 has been implicated in the regulation of the actin cytoskeleton and T cell receptor (TCR) signaling, which are crucial for normal thymocyte development and maturation. Additionally, the phosphorylation status of PAK2 can serve as a marker for cellular processes and can be detected using specific antibodies designed to recognize the phosphorylated form of the protein.

免疫印迹
- WB result of Phospho-PAK2 (Ser20) Recombinant Rabbit mAb
  Primary antibody: Phospho-PAK2 (Ser20) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated HeLa whole cell lysate 20 µg
  Lane 2: HeLa treated with 100 ng/ml Calyculin A for 30 minutes whole cell lysate 20 µg
  Lane 3: untreated Jurkat whole cell lysate 20 µg
  Lane 4: Jurkat strave overnight, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 58 kDa
  Observed MW: 62 kDa
- WB result of Phospho-PAK2 (Ser20) Recombinant Rabbit mAb
  Primary antibody: Phospho-PAK2 (Ser20) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
  Lane 2: NIH/3T3 strave for 3 hours, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 58 kDa
  Observed MW: 62 kDa
- WB result of Phospho-PAK2 (Ser20) Recombinant Rabbit mAb
  Primary antibody: Phospho-PAK2 (Ser20) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated C6 whole cell lysate 20 µg
  Lane 2: C6 treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 58 kDa
  Observed MW: 62 kDa
免疫沉淀
- Phospho-PAK2 (Ser20) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-PAK2 (Ser20) in 0.4 mg NIH/3T3 strave for 3 hours, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate.
  Western blot was performed on the immunoprecipitate using Phospho-PAK2 (Ser20) Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: NIH/3T3 strave for 3 hours, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg (Input)
  Lane 2: Phospho-PAK2 (Ser20) Rabbit mAb IP in NIH/3T3 strave for 3 hours, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in NIH/3T3 strave for 3 hours, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate
  Predicted MW: 58 kDa
  Observed MW: 62 kDa
免疫细胞化学
- ICC analysis of NIH/3T3 cells starved for 3 hours then treated with Calyculin A (100nM) for 30 minutes (top panel) and NIH/3T3 cells starved for 3 hours then untreated with Calyculin A (100nM) for 30 minutes (below panel). Anti- Phospho-PAK2 (Ser20) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).