Phospho-Syk (Tyr525/526) Recombinant Rabbit mAb (S-1245-113)

Tyrosine-protein kinase SYK,Spleen tyrosine kinase,p72-Syk

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1036

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Phospho-Syk (Tyr525/526)
分子别名

Tyrosine-protein kinase SYK; Spleen tyrosine kinase; p72-Syk
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Cell membrane
Accession

P43405
克隆号

S-1245-113
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Hu
阳性样本

Ramos starve 24 hours, then treated with 10 mM pervanadate for 30 minutes
预测反应种属
(反应种属缩写表)

Ms, Rt, Pg
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000-1:5000 Hu
IP 1:50 Hu
ICC 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000-1:5000	Hu
IP	1:50	Hu
ICC	1:500	Hu

背景介绍

Phospho-Syk (Tyr525/526) refers to the phosphorylation of the tyrosine kinase Syk at the tyrosine residues 525 and 526. This post-translational modification is crucial for the activation of Syk, which plays a significant role in various signaling pathways, particularly those involved in immune cell activation and function. Syk is a non-receptor tyrosine kinase that is involved in the regulation of immune responses, including B cell receptor signaling, Fc receptor signaling, and cytokine receptor signaling. Phosphorylation at Tyr525/526 is thought to be important for the full activation of Syk and its downstream signaling events, which can include the activation of other kinases such as phospholipase C gamma 2 (PLCγ2) and the mitogen-activated protein kinases (MAPKs).

免疫印迹
- WB result of Phospho-Syk (Tyr525/526) Recombinant Rabbit mAb
  Primary antibody: Phospho-Syk (Tyr525/526) Recombinant Rabbit mAb at 1/5000 dilution
  Lane 1: untreated Ramos whole cell lysate 20 µg
  Lane 2: Ramos starve 24 hours, then treated with 10 mM pervanadate for 30 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 72 kDa
  Observed MW: 72 kDa
免疫沉淀
- Phospho-SYK(Tyr525/Tyr526) Recombinant Rabbit mAb (S-1245-113) at 1/50 dilution (1 µg) immunoprecipitating Phospho-SYK(Tyr525/Tyr526) in 0.4 mg Ramos+serum starvation(24) then pervanadate(10mM,30min) whole cell lysate.
  Western blot was performed on the immunoprecipitate using Phospho-SYK(Tyr525/Tyr526) Recombinant Rabbit mAb (S-1245-113) at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: Ramos+serum starvation(24) then pervanadate(10mM,30min) whole cell lysate 20 µg (Input)
  Lane 2: Phospho-SYK(Tyr525/Tyr526) Recombinant Rabbit mAb (S-1245-113) IP in Ramos+serum starvation(24) then pervanadate(10mM,30min) whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in Ramos+serum starvation(24) then pervanadate(10mM,30min) whole cell lysate
  Predicted MW: 72 kDa
  Observed MW: 72 kDa
免疫细胞化学
- ICC analysis of ramos cells serum-starved (24h) then treated with pervanadate (10mM,30min) (top panel) and ramos cells serum-starved (24h) untreated with pervanadate (10mM,30min) (below panel). Anti- Phospho-Syk (Tyr525/526) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).