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Rabbit抗原名称
PI3 Kinase p110 β分子别名
PI3-kinase subunit beta; PI3K-beta; PI3Kbeta; PtdIns-3-kinase subunit beta; PIK3CB; PIK3C1免疫原
Synthetic Peptide细胞定位
Nucleus, CytoplasmAccession
P42338克隆号
S-1193-115抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
K562, MCF7, HeLa, C2C12, mouse brain, C6, rat brain纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt ICC 1:50 Hu, Ms
PI3 Kinase p110β, also known as PIK3CB, is a catalytic subunit of the phosphoinositide 3-kinase (PI3K) family, which plays a crucial role in cellular processes such as growth, metabolism, and tumorigenesis. This isoform, along with p110α, generates lipid second messengers that initiate multiple signal transduction cascades upon receptor activation. In the context of non-small cell lung cancer (NSCLC), p110β overexpression is associated with clinical significance and specific mechanisms. High expression rates of p110β in NSCLC have been reported to be around 41.8%. Its overexpression is particularly linked to the loss of PTEN, a negative regulator of the PI3K pathway, and is negatively correlated with EGFR mutations. This suggests that p110β may play a role in tumors where PTEN is deficient, potentially driving tumor survival and progression. Moreover, studies have shown that p110β has kinase-independent functions in regulating cell proliferation and trafficking.
免疫印迹
WB result of PI3 Kinase p110β Recombinant Rabbit mAb
Primary antibody: PI3 Kinase p110 β Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: K562 whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 123 kDa
Observed MW: 120 kDaWB result of PI3 Kinase p110β Recombinant Rabbit mAb
Primary antibody: PI3 Kinase p110 β Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 123 kDa
Observed MW: 120 kDaWB result of PI3 Kinase p110β Recombinant Rabbit mAb
Primary antibody: PI3 Kinase p110 β Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 123 kDa
Observed MW: 120 kDa
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- PI3 Kinase p110β antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in C2C12 cells. Anti- PI3 Kinase p110β antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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