Histone H4 (acetyl K16) Recombinant Rabbit mAb (S-1482-12)

H4K16ac

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0983

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Histone H4 (acetyl K16)
分子别名

H4K16ac
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

P62805
克隆号

S-1482-12
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

乙酰化
反应种属 ?

Hu, Ms, Rt
阳性样本

HeLa, NIH/3T3
预测反应种属
(反应种属缩写表)

Pl, Dr, Av
纯化方式

Protein A
浓度

0.5 mg/ml
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ChIP

IHC-P ?

WB
稀释度

应用稀释度推荐种属

Dot Blot 1:1000
WB 1:1000 Hu, Ms
IHC-P 1:500 Ms, Rt
ChIP 1:20~1:50 Hu

应用	稀释度	推荐种属
Dot Blot	1:1000
WB	1:1000	Hu, Ms
IHC-P	1:500	Ms, Rt
ChIP	1:20~1:50	Hu

背景介绍

Histone H4 acetylation at lysine 16 (H4K16ac) is a unique histone modification that plays a significant role in the regulation of chromatin structure and gene expression. This modification is catalyzed by the enzyme MOF (MYST-family lysine acetyltransferase), which is evolutionarily conserved and has been implicated in a variety of cellular processes, including the regulation of metabolism, cell cycle control, and cellular responses to DNA damage. It is the only known acetylation mark that is maintained across generations, suggesting its importance in epigenetic inheritance and gene regulation. In the context of metabolism, H4K16ac has been linked to the control of central carbon metabolism and diet-induced obesity in mice. Mice with reduced levels of MOF, the enzyme responsible for H4K16ac, show altered glucose assimilation, impaired insulin response, and resistance to weight gain when fed a high-fat diet. This is associated with changes in the transcriptional regulation of genes involved in glucose uptake and lipid storage in white adipose tissue. Moreover, H4K16ac has been implicated in the regulation of mitochondrial function and ciliary biogenesis.

免疫印迹
- WB result of Histone H4 (acetyl K16) Recombinant Rabbit mAb
  Primary antibody: Histone H4 (acetyl K16) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated HeLa whole cell lysate 20 µg
  Lane 2: HeLa treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 11 kDa
  Observed MW: 12 kDa
- WB result of Histone H4 (acetyl K16) Recombinant Rabbit mAb
  Primary antibody: Histone H4 (acetyl K16) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
  Lane 2: NIH/3T3 treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 11 kDa
  Observed MW: 12 kDa
斑点杂交
- Dot blot result of Histone H4 (acetyl K16) Recombinant Rabbit mAb
  Lane 1: H4K16ac modified peptide
  Lane 2: H4K16 unmodified peptide
  Primary antibody: Histone H4 (acetyl K16) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  This blot was developed with high sensitivity substrate
免疫组化
- IHC shows positive staining in paraffin-embedded mouse lung. Anti- Histone H4 (acetyl K16) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse testis. Anti- Histone H4 (acetyl K16) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat kidney. Anti- Histone H4 (acetyl K16) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat stomach. Anti- Histone H4 (acetyl K16) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ChIP
- Chromatin immunoprecipitation (ChIP) was
  performed on HeLa+TSA (500ng/ml,4h) (+) cells
  cross - linked with 1% formaldehyde for 10 min,
  then chromatin was fragmented by sonication.
  Parallel reactions used Histone H4 (acetyl K16)
  Recombinant Rabbit mAb (S-1482-12) and Rabbit
  mAb IgG Isotype Control (SDT-R173) at 1:50 for
  immunoprecipitation.
  Post - immunoprecipitation, both samples
  were washed, eluted, and cross - links
  reversed. Purified DNA was analyzed by qPCR.
  qPCR (%input: immunoprecipitated DNA/input DNA)
  showed the enrichment of RPL30, GAPDH, MYOD1, AFM,
  SAT-α and SAT-2 in Histone H4 (acetyl K16) Recombinant
  Rabbit mAb (S-1482-12)-immunoprecipitated sample.