Phospho-PTEN (S380) Recombinant Rabbit mAb (S-1165-163)

MMAC1,TEP1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0977

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Phospho-PTEN (S380)
分子别名

MMAC1; TEP1
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

P60484
克隆号

S-1165-163
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Dg, Xe
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

ICC ?

WB

IP
稀释度

应用稀释度推荐种属

Dot Blot 1:1000
WB 1:1000 Hu, Ms, Rt
IP 1:50 Hu
ICC 1:500 Hu, Ms

应用	稀释度	推荐种属
Dot Blot	1:1000
WB	1:1000	Hu, Ms, Rt
IP	1:50	Hu
ICC	1:500	Hu, Ms

背景介绍

PTEN, or phosphatase and tensin homolog, is a tumor suppressor protein that plays a crucial role in regulating cell growth, survival, and migration by antagonizing the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway. Phosphorylation of PTEN is an important post-translational modification that can affect its stability, subcellular localization, and enzymatic activity. The phosphorylation at S380, along with other residues such as T382 and T383, has been shown to be critical for the regulation of PTEN's function. Phosphorylation at these sites can lead to decreased stability of the PTEN protein, which in turn can affect its ability to suppress tumorigenesis. This post-translational modification can also influence the interaction of PTEN with other cellular proteins, impacting its downstream signaling effects. In the context of cancer, the phosphorylation state of PTEN is closely monitored as it can serve as a potential biomarker for prognosis and therapeutic response. Dysregulation of PTEN phosphorylation, including the S380 site, has been implicated in various cancers, suggesting its importance in tumor development and progression.

免疫印迹
- WB result of Phospho-PTEN (S380) Recombinant Rabbit mAb
  Primary antibody: Phospho-PTEN (S380) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: phosphatase treated HeLa whole cell lysate 20 µg
  Lane 2: HeLa whole cell 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 47 kDa
  Observed MW: 54 kDa
- WB result of Phospho-PTEN (S380) Recombinant Rabbit mAb
  Primary antibody: Phospho-PTEN (S380) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: phosphatase treated NIH/3T3 whole cell lysate 20 µg
  Lane 2: HeLa whole cell 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 47 kDa
  Observed MW: 54 kDa
- WB result of Phospho-PTEN (S380) Recombinant Rabbit mAb
  Primary antibody: Phospho-PTEN (S380) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: phosphatase treated C6 whole cell lysate 20 µg
  Lane 2: C6 whole cell 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 47 kDa
  Observed MW: 54 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling Phospho-PTEN (S380) antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) labelling Phospho-PTEN (S380) antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
- Phospho-PTEN (S380) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-PTEN (S380) in 0.4 mg HeLa whole cell lysate.
  Western blot was performed on the immunoprecipitate using Phospho-PTEN (S380) Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: HeLa whole cell lysate 20 µg (Input)
  Lane 2: Phospho-PTEN (S380) Rabbit mAb IP in HeLa whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
  Predicted MW: 47 kDa
  Observed MW: 54 kDa
斑点杂交
- Dot blot result of Phospho-PTEN (S380) Recombinant Rabbit mAb
  Lane 1: PTEN (S380) phospho peptide
  Lane 2: PTEN unmodified peptide
  Primary antibody: Phospho-PTEN (S380) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti- Phospho-PTEN (S380) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
- ICC shows positive staining in NIH/3T3 cells. Anti- Phospho-PTEN (S380) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).