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KDM1/LSD1 Recombinant Rabbit mAb (S-1280-80)

Lysine-specific histone demethylase 1A,BRAF35-HDAC complex protein BHC110,Flavin-containing amine oxidase domain-containing protein 2,[histone H3]-dimethyl-L-lysine(4) FAD-dependent demethylase 1A,AOF2,KDM1,KIAA0601

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0927
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产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    KDM1/LSD1

  • 分子别名

    Lysine-specific histone demethylase 1A; BRAF35-HDAC complex protein BHC110; Flavin-containing amine oxidase domain-containing protein 2; [histone H3]-dimethyl-L-lysine(4) FAD-dependent demethylase 1A; AOF2; KDM1; KIAA0601

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    O60341

  • 克隆号

    S-1280-80

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 预测反应种属
    (反应种属缩写表)

    Fu

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    ICFCM

    ChIP

    IHC-P ?

    ICC ?

    WB

    IP

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms, Rt
    IP 1:50 Hu
    IHC-P 1:500 Hu, Ms, Rt
    ICC 1:500 Hu
    ICFCM 1:50 Hu
    ChIP 1:20-1:50 Hu
背景介绍

  • KDM1, also known as Lysine-specific histone demethylase 1A, is an enzyme that plays a crucial role in the regulation of gene expression. It is involved in the process of demethylation, which is the removal of methyl groups from lysine residues on histone proteins. This modification of histones can lead to changes in the accessibility of DNA to the transcription machinery, thereby affecting whether a gene is turned on or off.

  • 免疫印迹

    • WB result of KDM1/LSD1 Recombinant Rabbit mAb
      Primary antibody: KDM1/LSD1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: MCF7 whole cell lysate 20 µg
      Lane 3: Jurkat whole cell lysate 20 µg
      Lane 4: HCT 116 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 93 kDa
      Observed MW: 110 kDa

    • WB result of KDM1/LSD1 Recombinant Rabbit mAb
      Primary antibody: KDM1/LSD1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 93 kDa
      Observed MW: 110 kDa

    • WB result of KDM1/LSD1 Recombinant Rabbit mAb
      Primary antibody: KDM1/LSD1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 93 kDa
      Observed MW: 110 kDa

  • 流式分析

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling KDM1/LSD1 antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

  • 免疫沉淀

    • KDM1/LSD1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating KDM1/LSD1 in 0.4 mg HeLa whole cell lysate.
      Western blot was performed on the immunoprecipitate using KDM1/LSD1 Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/1000 dilution.
      Lane 1: HeLa whole cell lysate 20 µg (Input)
      Lane 2: KDM1/LSD1 Rabbit mAb IP in HeLa whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
      Predicted MW: 93 kDa
      Observed MW: 110 kDa

  • 染色质免疫沉淀

    • Chromatin immunoprecipitation (ChIP) was performed on HeLa cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used KDM1/LSD1 Recombinant Rabbit mAb (S-1280-80) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
      Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
      qPCR showed the enrichment of VIM, SPI-1 and SAT-α in KDM1/LSD1 Recombinant Rabbit mAb (S-1280-80) -immunoprecipitated sample.

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti- KDM1/LSD1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human stomach. Anti- KDM1/LSD1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti- KDM1/LSD1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti- KDM1/LSD1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti- KDM1/LSD1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat kidney. Anti- KDM1/LSD1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti- KDM1/LSD1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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