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宿主来源
Rabbit抗原名称
CREB (phospho S133)分子别名
Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1免疫原
Synthetic Peptide细胞定位
NucleusAccession
P16220-1克隆号
S-1100-102抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Hu, Ms, Rt预测反应种属
(反应种属缩写表)Bv纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ChIP
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 Dot Blot 1:1000 WB 1:1000 Hu IHC-P 1:200 Hu, Ms, Rt ChIP 1:20-1:50 Hu
CREB (cAMP response element-binding protein) is a well-known transcription factor that plays a pivotal role in the regulation of gene expression in response to various stimuli, including neuronal activity, hormones, and growth factors. Phosphorylation of CREB at serine 133 (Ser133) is a critical event for its activation, as it enhances the binding of CREB to the cAMP response element (CRE), leading to the transcription of target genes involved in processes such as learning, memory, and cell survival. The phosphorylation of CREB at Ser133 is typically catalyzed by various protein kinases, including protein kinase A (PKA), calcium/calmodulin-dependent protein kinase IV (CaMKIV), and mitogen-activated protein kinase (MAPK). This phosphorylation event is a robust indicator of neuronal activation and synaptic plasticity, making it a widely studied marker in neurobiological research.
免疫印迹
WB result of CREB (phospho S133) Recombinant Rabbit mAb
Primary antibody: CREB (phospho S133) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 nM Calyculin A for 15 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 45 kDa
斑点杂交
Dot blot result of CREB (phospho S133) Recombinant Rabbit mAb
Lane 1: CREB (S133) phospho peptide
Lane 2: CREB unmodified peptide
Primary antibody: CREB (phospho S133) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti- CREB (phospho S133) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti- CREB (phospho S133) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti- CREB (phospho S133) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti- CREB (phospho S133) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti- CREB (phospho S133) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ChIP
Chromatin immunoprecipitation (ChIP) was performed on HeLa cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactionsused CREB (phospho S133) Recombinant Rabbit mAb (S-1100-102) and IgG IsotypeControl (SDT-R173) at 1:50 for immunoprecipitation.
Post-immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
qPCR showed the enrichment of ALS2, NR4A3 and SAT-α in
CREB (phospho S133) Recombinant Rabbit mAb (S-1100-102)-immunoprecipitated
sample.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高
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