LKB1 Recombinant Rabbit mAb (S-1264-31)

Serine/threonine-protein kinase STK11,Liver kinase B1 (LKB1,hLKB1),Renal carcinoma antigen NY-REN-19,STK11,PJS

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0833

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

LKB1
分子别名

Serine/threonine-protein kinase STK11; Liver kinase B1 (LKB1; hLKB1); Renal carcinoma antigen NY-REN-19; STK11; PJS
免疫原

Recombinant Protein
细胞定位

Nucleus
Accession

Q15831
克隆号

S-1264-31
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

IHC-P ?

ICC ?

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Rt
IP 1:50 Rt
IHC-P 1:500 Hu, Ms, Rt
ICC 1:500 Hu
ICFCM 1:50 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Rt
IP	1:50	Rt
IHC-P	1:500	Hu, Ms, Rt
ICC	1:500	Hu
ICFCM	1:50	Hu

背景介绍

LKB1 is a serine/threonine kinase that belongs to the AMPK-related kinase family. LKB1 functions as a heterotrimeric complex with STRAD (STE20-related adaptor) and MO25 (mouse protein 25) proteins, which are necessary for its stability and full kinase activity. LKB1 is mutated in a variety of cancers, including Peutz-Jeghers syndrome (PJS), a rare autosomal dominant disorder characterized by benign gastrointestinal polyps and increased risk of malignancies. Inactivation of LKB1 leads to enhanced cell proliferation, altered energy metabolism, and resistance to apoptosis, promoting tumor development. Restoring LKB1 function has been shown to suppress tumor growth and metastasis in various cancer models. LKB1 is also a master kinase that activates multiple downstream kinases involved in cellular energy metabolism, including AMP-activated protein kinase (AMPK) and 12 related kinases (MARKs).

免疫印迹
- WB result of LKB1 Recombinant Rabbit mAb
  Primary antibody: LKB1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: K562 whole cell lysate 20 µg
  Lane 3: PANC-1 whole cell lysate 20 µg
  Lane 4: 293T whole cell lysate 20 µg
  Negative control: HeLa whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 49 kDa
  Observed MW: 55 kDa
- WB result of LKB1 Recombinant Rabbit mAb
  Primary antibody: LKB1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: rat testis lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 49 kDa
  Observed MW: 48, 55 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized K562 (Human chronic myelogenous leukemia lymphoblast) labelling LKB1 antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
- LKB1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating LKB1 in 0.4 mg rat testis lysate.
  Western blot was performed on the immunoprecipitate using LKB1 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: rat testis lysate 20 µg (Input)
  Lane 2: LKB1 Rabbit mAb IP in rat testis lysate
  Lane 3: Rabbit monoclonal IgG IP in rat testis lysate
  Predicted MW: 49 kDa
  Observed MW: 48, 55 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human testis. Anti- LKB1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse testis. Anti- LKB1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat testis. Anti- LKB1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in K562 cells. Anti-LKB1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).