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Rabbit抗原名称
LRRK2 (phospho S935)分子别名
Leucine-rich repeat serine/threonine-protein kinase 2, Dardarin, PARK8免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
Q5S007克隆号
S-966-37抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Hu预测反应种属
(反应种属缩写表)Ms, Pr, Rt, Pg纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 Dot Blot 1:1000 WB 1:1000
LRRK2, also known as Leucine-rich repeat kinase 2, is a large, multi-domain protein kinase that belongs to the ROCO (Ras of complex proteins) family. It is highly conserved across species and plays important roles in various cellular processes, including vesicle trafficking, autophagy, and cytoskeletal dynamics. Mutations in the LRRK2 gene have been linked to several neurodegenerative disorders, most notably Parkinson's disease. The phosphorylation of LRRK2 at S935 has been implicated in modulating its kinase activity and may contribute to the pathogenesis of Parkinson's disease and other LRRK2-related disorders.
免疫印迹
WB result of LRRK2 (phospho S935) Recombinant Rabbit mAb
Primary antibody: LRRK2 (phospho S935) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: GFP-His LRRK2 lysate 5 ug
Lane 2: GFP-His LRRK2 S910A lysate 5 ug
Lane 3: GFP-His LRRK2 S935A lysate 5 ug
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 313 kDa
Observed MW: 280 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高
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