Histone H3 (mono methyl K9) Recombinant Rabbit mAb (S-850-153)

H3K9me1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0822

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Histone H3 (mono methyl K9)
分子别名

H3K9me1
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

P68431
克隆号

S-850-153
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

甲基化
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Fu, Pl, Ys, SeUr, Dr, C.el, Hm, Or, Bv, Xe
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

ChIP

ICC ?

WB
稀释度

应用稀释度推荐种属

Dot Blot 1:1000
WB 1:1000 Hu, Ms, Rt
ICC 1:500 Hu
ICFCM 1:500 Hu
ChIP 1:20-1:50 Hu

应用	稀释度	推荐种属
Dot Blot	1:1000
WB	1:1000	Hu, Ms, Rt
ICC	1:500	Hu
ICFCM	1:500	Hu
ChIP	1:20-1:50	Hu

背景介绍

H3K9me1 refers to the monomethylation of the ninth lysine residue on histone H3. This modification can either activate or repress gene expression, depending on the cellular context, developmental stage, and environmental cues. In some instances, H3K9me1 may contribute to maintaining an open chromatin state, facilitating the binding of transcription factors to DNA and thereby activating gene transcription. Alternatively, it can associate with repressive complexes, leading to chromatin condensation and gene repression. The levels of H3K9me1 are tightly regulated by a balance of enzymatic activities, including methyltransferases (such as SETDB1 and G9a) that add methyl groups and demethylases (like LSD1) that remove them. These enzymes precisely control the extent of H3K9me1 modification, thereby modulating gene expression and cellular behaviors.

免疫印迹
- WB result of Histone H3 (mono methyl K9) Recombinant Rabbit mAb
  Primary antibody: Histone H3 (mono methyl K9) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: MCF7 whole cell lysate 20 µg
  Lane 3: Jurkat whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 15 kDa
  Observed MW: 15 kDa
- WB result of Histone H3 (mono methyl K9) Recombinant Rabbit mAb
  Primary antibody: Histone H3 (mono methyl K9) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: Neuro-2a whole cell lysate 20 µg
  Lane 2: NIH/3T3 whole cell lysate 20 µg
  Lane 3: C2C12 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 15 kDa
  Observed MW: 15 kDa
- WB result of Histone H3 (mono methyl K9) Recombinant Rabbit mAb
  Primary antibody: Histone H3 (mono methyl K9) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 15 kDa
  Observed MW: 15 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling Histone H3 (mono methyl K9) antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
斑点杂交
- Dot blot result of Histone H3 (mono methyl K9) Recombinant Rabbit mAb
  Lane 1: H3K9me1 peptide
  Lane 2: H3K9me2 peptide
  Lane 3: H3K9me3 peptide
  Lane 4: H3K4me1 peptide
  Lane 5: H3K9un peptide
  Primary antibody: Histone H3 (mono methyl K9) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti- Histone H3 (mono methyl K9) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ChIP
- Chromatin immunoprecipitation (ChIP) was performed on HeLa cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Histone H3 (mono methyl K9) Recombinant Rabbit mAb (S-850-153) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
  Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
  qPCR (%input: immunoprecipitated DNA/input DNA)
  showed the enrichment of RPL30, GAPDH, MYOD1,
  AFM, SAT-α and SAT-2 in Histone H3 (mono methyl K9) Recombinant
  Rabbit mAb (S-850-153)-immunoprecipitated sample.