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p90RSK Recombinant Rabbit mAb (S-1024-26)

Ribosomal protein S6 kinase alpha-1,S6K-alpha-1,90 kDa ribosomal protein S6 kinase 1 (p90-RSK 1,p90RSK1,p90S6K),MAP kinase-activated protein kinase 1a (MAPK-activated protein kinase 1a,MAPKAP kinase 1a,MAPKAPK-1a),Ribosomal S6 kinase 1 (RSK-1),RPS6KA1,MAPKAPK1A,RSK1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0782

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

p90RSK
分子别名

Ribosomal protein S6 kinase alpha-1, S6K-alpha-1, 90 kDa ribosomal protein S6 kinase 1 (p90-RSK 1; p90RSK1; p90S6K), MAP kinase-activated protein kinase 1a (MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a), Ribosomal S6 kinase 1 (RSK-1), RPS6KA1, MAPKAPK1A, RSK1
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

Q15418
克隆号

S-1024-26
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IHC-P 1:500 Hu, Ms, Rt
IP 1:50 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IHC-P	1:500	Hu, Ms, Rt
IP	1:50	Hu

背景介绍

p90RSK, also known as RSK1 or RPS6KA1 (ribosomal protein S6 kinase alpha-1), belongs to the serine/threonine kinase family. It is a widely expressed kinase that is involved in regulating various cellular activities such as cell growth, proliferation, and survival. As a downstream effector molecule in the mitogen-activated protein kinase (MAPK) pathway, p90RSK plays a crucial role.

免疫印迹
- WB result of p90RSK Recombinant Rabbit mAb
  Primary antibody: p90RSK Recombinant mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: MCF-7 whole cell lysate 20 µg
  Lane 3: A431 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 83 kDa
  Observed MW: 90 kDa
- WB result of p90RSK Recombinant Rabbit mAb
  Primary antibody: p90RSK Recombinant mAb at 1/1000 dilution
  Lane 1: mouse lung lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 83 kDa
  Observed MW: 90 kDa
- WB result of p90RSK Recombinant Rabbit mAb
  Primary antibody: p90RSK Recombinant mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 83 kDa
  Observed MW: 90 kDa
免疫沉淀
- p90RSK Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating p90RSK in 0.4 mg HeLa whole cell lysate.
  Western blot was performed on the immunoprecipitate using p90RSK Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: HeLa whole cell lysate 20 µg (Input)
  Lane 2: p90RSK Rabbit mAb IP in HeLa whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
  Predicted MW: 83 kDa
  Observed MW: 90 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human colon. Anti- p90RSK antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human kidney. Anti- p90RSK antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human tonsil. Anti- p90RSK antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti- p90RSK antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse kidney. Anti- p90RSK antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat colon. Anti- p90RSK antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.