分享产品介绍 评论(0)
宿主来源
Mouse分子别名
p‑Tyr, pY, 酪氨酸磷酸化克隆号
S-R433-1抗体类型
Mouse mAb抗体同种型
IgG2b,k反应种属 ?
Hu, Ms纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 Dot Blot 1:2000 WB 1:1000 ICC 1:500
Tyrosine phosphorylation refers to the addition of a phosphate group to the hydroxyl oxygen of the tyrosine amino acid residue in a protein. This process is catalyzed by specific kinases, such as protein tyrosine kinases (PTKs), which transfer the gamma phosphate group of ATP to the tyrosine hydroxyl group. Tyrosine phosphorylation is involved in the regulation of protein function, activity, and localization. It plays a key role in signal transduction pathways, mediating cellular responses to extracellular stimuli.
免疫印迹
WB result of Phosphotyrosine Recombinant Mouse mAb
Primary antibody: Phosphotyrosine Recombinant Mouse mAb at 1/1000 dilution
Lane 1: untreated A431 whole cell lysate 20 µg
Lane 2: A431 treated with 50 mM Pervanadate for 5 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: MultipleWB result of Phosphotyrosine Recombinant Mouse mAb
Primary antibody: Phosphotyrosine Recombinant Mouse mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 10 mM Pervanadate for 20 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple
斑点杂交
Dot blot result of Phosphotyrosine Mouse mAb
Lane 1: Modified peptide library (Phospho-Serine)
Lane 2: Modified peptide library (Phospho-Threonine)
Lane 3: Modified peptide library (Phospho-Tyrosine)
Lane 4: Unmodified peptide library (Serine)
Lane 5: Unmodified peptide library (Threonine)
Lane 6: Unmodified peptide library (Tyrosine)
Primary antibody: Phosphotyrosine Mouse mAb at 1/2000 dilution
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫细胞化学
ICC analysis of A431 cells treated with Pervanadate (50mM, 5min) (top panel) and A431 cells untreated with Pervanadate (50mM, 5min) (below panel). Anti- Phosphotyrosine antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
评论(0)