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KDM1B Recombinant Rabbit mAb (S-993-8)

Lysine-specific histone demethylase 2,Flavin-containing amine oxidase domain-containing protein 1,Lysine-specific histone demethylase 1B,AOF1,C6orf193,LSD2

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0746

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
分子别名

Lysine-specific histone demethylase 2, Flavin-containing amine oxidase domain-containing protein 1, Lysine-specific histone demethylase 1B, AOF1, C6orf193, LSD2
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

Q8NB78
克隆号

S-993-8
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

2 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
ICC 1:400-1:2000 Hu
ICFCM 1:200 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
ICC	1:400-1:2000	Hu
ICFCM	1:200	Hu

背景介绍

KDM1B, also known as LSD2 or Aof1, is an enzyme that plays crucial roles in various biological processes. It has FAD-binding activity and FAD-dependent demethylase activity specific to histone H3-di/monomethyl-lysine-4. It is involved in the regulation of DNA methylation, particularly upstream of spermatogenesis, histone H3-K4 demethylation, and the regulation of gene expression by genetic imprinting.

免疫印迹
- WB result of KDM1B Recombinant Rabbit mAb
  Primary antibody: KDM1B Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HepG2 whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Lane 3: HCT 116 whole cell lysate 20 µg
  Weak expression: HepG2 whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 92 kDa
  Observed MW: 92 kDa
  This blot was developed with high sensitivity substrate
- WB result of KDM1B Recombinant Rabbit mAb
  Primary antibody: KDM1B Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: mouse thymus lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 92 kDa
  Observed MW: 92 kDa
- WB result of KDM1B Recombinant Rabbit mAb
  Primary antibody: KDM1B Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Lane 2: rat thymus lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 92 kDa
  Observed MW: 92 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling KDM1B antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-KDM1B antibody was used at 1/400 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).