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宿主来源
Rabbit抗原名称
Acetyllysine克隆号
S-R373抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Species Independent纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
IP
稀释度
应用 稀释度 WB 1:500 IP 1:20
Acetyllysine (or acetylated lysine) is an acetyl-derivative of the amino acid lysine. In proteins, the acetylation of lysine residues is an important mechanism of epigenetics. It functions by regulating the binding of histones to DNA in nucleosomes and thereby controlling the expression of genes on that DNA. Non-histone proteins are acetylated as well. Unlike the functionally similar methyllysine, acetyllysine does not carry a positive charge on its side chain. Histone acetyltransferases (HATs) catalyze the addition of acetyl groups from acetyl-CoA onto certain lysine residues of histones and non-histone proteins. Histone deacetylases (HDACs) catalyze the removal of acetyl groups from acetylated lysines.
免疫印迹
WB result of Acetyllysine Rabbit mAb
Primary antibody: Acetyllysine Rabbit mAb at 1/500 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple
免疫沉淀
Acetyllysine Recombinant Rabbit mAb at 1/20 dilution (2.5 µg) immunoprecipitating Acetyllysine in 0.4 mg HeLa+ Sodium Butyrate (30mM, 4hr) whole cell lysate.
Western blot was performed on the immunoprecipitate using Acetyllysine Recombinant Rabbit mAb at 1/500 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa+ Sodium Butyrate (30mM, 4hr) whole cell lysate 20 µg (Input)
Lane 2: Acetyllysine Recombinant Rabbit mAb IP in HeLa+ Sodium Butyrate (30mM, 4hr) whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa+ Sodium Butyrate (30mM, 4hr) whole cell lysate
Predicted MW: Multiple
Observed MW: Multiple
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