分享产品介绍 评论(0)
宿主来源
Rabbit抗原名称
Acetyllysine分子别名
Kac, 乙酰化克隆号
S-R373抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Species Independent纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
IP
稀释度
应用 稀释度 Dot Blot 1:500 WB 1:500 IP 1:20
Acetyllysine (or acetylated lysine) is an acetyl-derivative of the amino acid lysine. In proteins, the acetylation of lysine residues is an important mechanism of epigenetics. It functions by regulating the binding of histones to DNA in nucleosomes and thereby controlling the expression of genes on that DNA. Non-histone proteins are acetylated as well. Unlike the functionally similar methyllysine, acetyllysine does not carry a positive charge on its side chain. Histone acetyltransferases (HATs) catalyze the addition of acetyl groups from acetyl-CoA onto certain lysine residues of histones and non-histone proteins. Histone deacetylases (HDACs) catalyze the removal of acetyl groups from acetylated lysines.
免疫印迹
WB result of S-RMabMix™ Acetyllysine Rabbit mAb
Primary antibody: S-RMabMix™ Acetyllysine Rabbit mAb at 1/500 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple
Exposure time: 180 s
斑点杂交
Dot blot result of S-RMabMix™ Acetyllysine Rabbit mAb
Lane 1: Modified peptide library (Acetyl lysine)
Lane 2: Unmodified peptide library control
Lane 3: Modified peptide library (Fumaryl lysine)
Lane 4: Modified peptide library (Crotonyl lysine)
Lane 5: Modified peptide library (Succinyl lysine)
Lane 6: Modified peptide library (Butyryl lysine)
Lane 7: Modified peptide library (L-lactyl lysine)
Primary antibody: S-RMabMix™ Acetyllysine Rabbit mAb at 1/500 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
评论(0)