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GLUT4 Recombinant Rabbit mAb (S-584-50)

Solute carrier family 2 facilitated glucose transporter member 4,Glucose transporter type 4 insulin-responsive (GLUT-4),SLC2A4

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0602

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
分子别名

Solute carrier family 2 facilitated glucose transporter member 4, Glucose transporter type 4 insulin-responsive (GLUT-4), SLC2A4
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Cell membrane
Accession

P14672
克隆号

S-584-50
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000 Ms, Rt
IP 1:50 Ms
IHC-P 1:500-1:2000 Hu, Ms, Rt

应用	稀释度	推荐种属
WB	1:1000	Ms, Rt
IP	1:50	Ms
IHC-P	1:500-1:2000	Hu, Ms, Rt

背景介绍

GLUT4 is the insulin-regulated glucose transporter found primarily in adipose tissues and striated muscle (skeletal and cardiac). At the cell surface, GLUT4 permits the facilitated diffusion of circulating glucose down its concentration gradient into muscle and fat cells. Once within cells, glucose is rapidly phosphorylated by glucokinase in the liver and hexokinase in other tissues to form glucose-6-phosphate, which then enters glycolysis or is polymerized into glycogen. In addition, recent reports demonstrated the presence of GLUT4 gene in central nervous system such as the hippocampus. Moreover, impairment in insulin-stimulated trafficking of GLUT4 in the hippocampus result in decreased metabolic activities and plasticity of hippocampal neurons, which leads to depressive like behaviour and cognitive dysfunction.

免疫印迹
- WB result of GLUT4 Rabbit mAb
  Primary antibody: GLUT4 Rabbit mAb at 1/1000 dilution
  Lane 1: unboiled mouse heart lysate 20 µg
  Lane 2: unboiled mouse skeletal muscle lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 55 kDa
  Observed MW: 40~55 kDa
- WB result of GLUT4 Rabbit mAb
  Primary antibody: GLUT4 Rabbit mAb at 1/1000 dilution
  Lane 1: unboiled rat heart lysate 20 µg
  Lane 2: unboiled rat skeletal muscle lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 55 kDa
  Observed MW: 40~55 kDa
免疫沉淀
- GLUT4 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating GLUT4 in 0.4 mg mouse heart lysate.
  Western blot was performed on the immunoprecipitate using GLUT4 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: mouse heart lysate 20 µg (Input)
  Lane 2: GLUT4 Rabbit mAb IP in mouse heart lysate
  Lane 3: Rabbit monoclonal IgG IP in mouse heart lysate
  Predicted MW: 55 kDa
  Observed MW: 40~55 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-GLUT4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-GLUT4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-GLUT4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-GLUT4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse skeletal muscle. Anti-GLUT4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-GLUT4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.