Phospho-Akt (Ser473) Recombinant Rabbit mAb (S-763-7)

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0596

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Phospho-Akt (Ser473)
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

Q9Y243
克隆号

S-763-7
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

WB

IP
稀释度

应用稀释度

WB 1:1000
IP 1:50

应用	稀释度
WB	1:1000
IP	1:50

背景介绍

Akt, also known as Protein kinase B (PKB), is the collective name of a set of three serine/threonine-specific protein kinases that play key roles in multiple cellular processes such as glucose metabolism, apoptosis, cell proliferation, transcription, and cell migration. Once correctly positioned at the membrane via binding of PIP3, Akt can then be phosphorylated by its activating kinases, phosphoinositide-dependent kinase-1 (PDPK1 at threonine 308 in Akt1 and threonine 309 in Akt2) and the mammalian target of rapamycin complex 2 (mTORC2 at serine 473 (Akt1) and 474 (Akt2)). Activated Akt isoforms can then go on to activate or deactivate their myriad substrates (e.g. mTOR) via their kinase activity. The Akt kinases regulate cellular survival and metabolism by binding and regulating many downstream effectors, e.g. Nuclear Factor-κB, Bcl-2 family proteins, master lysosomal regulator TFEB and murine double minute 2 (MDM2).

免疫印迹
- WB result of Phospho-Akt (Ser473) Rabbit mAb
  Primary antibody: Phospho-Akt (Ser473) Rabbit mAb at 1/1000 dilution
  Lane 1: untreated Jurkat whole cell lysate 20 µg
  Lane 2: Jurkat treated with Calyculin A (100 nM, 30 mins) whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 56 kDa
  Observed MW: 60 kDa
- WB result of Phospho-Akt (Ser473) Rabbit mAb
  Primary antibody: Phospho-Akt (Ser473) Rabbit mAb at 1/1000 dilution
  Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
  Lane 2: NIH/3T3 treated with Calyculin A (100 nM, 30 mins) whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 56 kDa
  Observed MW: 60 kDa
- WB result of Phospho-Akt (Ser473) Rabbit mAb
  Primary antibody: Phospho-Akt (Ser473) Rabbit mAb at 1/1000 dilution
  Lane 1: untreated C6 whole cell lysate 20 µg
  Lane 2: C6 treated with Calyculin A (100 nM, 30 mins) whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 56 kDa
  Observed MW: 60 kDa
免疫沉淀
- Phospho-Akt (Ser473) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-Akt (Ser473) in 0.4 mg Jurkat treated with Calyculin A (100 nM, 30 mins) whole cell lysate.
  Western blot was performed on the immunoprecipitate using Phospho-Akt (Ser473) Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: Jurkat treated with Calyculin A (100 nM, 30 mins) whole cell lysate 20 µg (Input)
  Lane 2: Phospho-Akt (Ser473) Rabbit mAb IP in Jurkat treated with Calyculin A (100 nM, 30 mins) whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in Jurkat treated with Calyculin A (100 nM, 30 mins) whole cell lysate
  Predicted MW: 56 kDa
  Observed MW: 60 kDa
  This blot was developed with high sensitivity substrate
斑点杂交
- Dot blot result of Phospho-Akt (Ser473) Rabbit mAb
  Lane 1: Phospho-Akt (Ser473) peptide
  Lane 2: Akt unmodified peptide
  Primary antibody: Phospho-Akt (Ser473) Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution