Histone H3 (mono methyl K36) Rabbit polyclonal antibody

H3K36me

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0525

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Histone H3 (mono methyl K36)
分子别名

H3K36me
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

P68431
抗体类型

Polyclonal antibody
抗体同种型

IgG
翻译后修饰类型

甲基化
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Fu, Pl, Ys, SeUr, Dr, C.el, Hm, Or, Bv, Xe
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ChIP

ICC ?

WB
稀释度

应用稀释度推荐种属

Dot Blot 1:1000
WB 1:1000 Hu, Ms, Rt
ICC 1:500 Hu
ChIP 1:20-1:50 Hu

应用	稀释度	推荐种属
Dot Blot	1:1000
WB	1:1000	Hu, Ms, Rt
ICC	1:500	Hu
ChIP	1:20-1:50	Hu

背景介绍

H3K36me is an epigenetic modification to the DNA packaging protein Histone H3, specifically, the mono-methylation at the 36th lysine residue of the histone H3 protein. The methylation of H3K36 has particularly had effects in transcriptional repression, alternative splicing, dosage compensation, DNA replication and repair, DNA methylation, and the transmission of the memory of gene expression from parents to offspring during development.

免疫印迹
- WB result of Histone H3 (mono methyl K36) Rabbit pAb
  Primary antibody: Histone H3 (mono methyl K36) pAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: MCF7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 17 kDa
  Observed MW: 17 kDa
  Exposure time: 180 s
- WB result of Histone H3 (mono methyl K36) Rabbit pAb
  Primary antibody: Histone H3 (mono methyl K36) pAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: Neuro-2a whole cell lysate 20 µg
  Lane 3: mouse liver lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 17 kDa
  Observed MW: 17 kDa
  Exposure time: 180 s
- WB result of Histone H3 (mono methyl K36) Rabbit pAb
  Primary antibody: Histone H3 (mono methyl K36) pAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Lane 2: PC-12 whole cell lysate 20 µg
  Lane 3: rat liver lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 17 kDa
  Observed MW: 17 kDa
  Exposure time: 180 s
斑点杂交
- Dot blot result of Histone H3 (mono methyl K36) Rabbit pAb
  Lane 1: H3 (mono methyl K36) peptide
  Lane 2: H3 (di methyl K36) peptide
  Lane 3: H3 (tri methyl K36) peptide
  Lane 4: H3 unmodified peptide
  Primary antibody: Histone H3 (mono methyl K36) Rabbit pAb at 1/1000 dilution
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Exposure time: 30 s
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti- Histone H3 (mono methyl K36) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
免疫荧光
- .
ChIP
- Chromatin immunoprecipitation (ChIP) was performed on HeLa cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Histone H3 (mono methyl K36) Rabbit polyclonal antibody and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
  Post-immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
  qPCR (%input: immunoprecipitated DNA/input DNA)
  showed the enrichment of RPL30, GAPDH, MYOD1,
  AFM, SAT-α and SAT-2 in Histone H3 (mono methyl K36)
  Rabbit polyclonal antibody - immunoprecipitated sample.