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Rabbit抗原名称
Collagen I (COL1A1)分子别名
Collagen alpha-1(I) chain免疫原
Synthetic Peptide细胞定位
Extracellular matrix, Secreted, Extracellular spaceAccession
P02452克隆号
S-463-3抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, ICC, WB反应种属 ?
Hu预测反应种属
(反应种属缩写表)Bv, Dg, Av纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:5000 |
| IHC | 1:1000 |
| ICC | 1:500 |
Collagen alpha-1 (I) chain (also known as Alpha-1 type I collagen) is encoded by the COL1A1 gene in human. Collagen is the major component of the extracellular matrix (ECM) and form the fibrils of tendons, ligaments, and bones. Type I collagen consists of two alpha I chains and one alpha 2 chain.
免疫印迹
WB result of Collagen I (COL1A1) Rabbit mAb
Primary antibody: Collagen I (COL1A1) Rabbit mAb at 1/5000 dilution
Lane 1: HFF whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 139 kDa
Observed MW: 220 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-Collagen I (COL1A1) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Collagen I (COL1A1) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HFF cells. Anti- Collagen I (COL1A1) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).
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