Terminal Deoxynucleotidyl Transferase (TdT) Recombinant Rabbit mAb (S-R245)

货号： S0B0331

Datasheet

价格： ￥600
规格：
25μl100μl500μl1ml
数量：

大包装询价

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产品规格

宿主来源

Rabbit
抗原名称

Terminal Deoxynucleotidyl Transferase (TdT)
分子别名

DNA nucleotidylexotransferase, Terminal addition enzyme, DNTT
细胞定位

Nucleus
Accession

P04053
克隆号

S-R245
抗体类型

Recombinant mAb
应用

ICC, WB, IP
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:1000
ICC	1:500
IP	1:50

背景介绍

Terminal deoxynucleotidyl transferase (TdT), also known as DNA nucleotidylexotransferase (DNTT) or terminal transferase, is a specialized DNA polymerase expressed in immature, pre-B, pre-T lymphoid cells, and acute lymphoblastic leukemia/lymphoma cells. TdT adds N-nucleotides to the V, D, and J exons of the TCR and BCR genes during antibody gene recombination, enabling the phenomenon of junctional diversity. The diversity introduced by TdT has played an important role in the evolution of the vertebrate immune system, significantly increasing the variety of antigen receptors that a cell is equipped with to fight pathogens.

免疫印迹
- WB result of Terminal Deoxynucleotidyl Transferase (TdT) Rabbit mAb
  Primary antibody: Terminal Deoxynucleotidyl Transferase (TdT) Rabbit mAb at 1/1000 dilution
  Lane 1: Jurkat whole cell lysate 20 µg
  Lane 2: MOLT-4 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 58 kDa
  Observed MW: 58 kDa
免疫沉淀
- Terminal Deoxynucleotidyl Transferase (TdT) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Terminal Deoxynucleotidyl Transferase (TdT) in 0.4 mg Jurkat whole cell lysate.
  Western blot was performed on the immunoprecipitate using Terminal Deoxynucleotidyl Transferase (TdT) Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: Jurkat whole cell lysate 20 µg (Input)
  Lane 2: Terminal Deoxynucleotidyl Transferase (TdT) Rabbit mAb IP in Jurkat whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in Jurkat whole cell lysate
  Predicted MW: 58 kDa
  Observed MW: 58 kDa
  （This blot was developed with high sensitivity substrate）
免疫细胞化学
- ICC shows positive staining in Molt-4 cells. Anti-Terminal Deoxynucleotidyl Transferase (TDT) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).