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宿主来源
Rabbit抗原名称
Phospho-Tyrosine分子别名
p‑Tyr, pY, 酪氨酸磷酸化克隆号
S-R207抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IP
稀释度
应用 稀释度 Dot Blot 1:500 WB 1:1000 IP 1:50 IHC 1:500
Tyrosine phosphorylation is the addition of a phosphate (PO43−) group to the amino acid tyrosine on a protein. It is one of the main types of protein phosphorylation. This transfer is made possible through enzymes called tyrosine kinases. Tyrosine phosphorylation is a key step in signal transduction and the regulation of enzymatic activity.
免疫印迹
WB result of Phosphotyrosine Rabbit mAb
Primary antibody: Phosphotyrosine Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with Sodium pervanadate (3 mM, 30 min) whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: multiple
Observed MW: multiple
Exposure time: 180 s
免疫沉淀
Phosphotyrosine Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-Tyrosine in 0.4 mg HeLa treated with Sodium pervanadate (3mM, 30min) whole cell lysate.
Western blot was performed on the immunoprecipitate using Phospho-Tyrosine Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: HeLa treated with Sodium pervanadate (3 mM, 30 min) whole cell lysate 20 µg (Input)
Lane 2: Phospho-Tyrosine Rabbit mAb IP in HeLa treated with Sodium pervanadate (3mM, 30min) whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa treated with Sodium pervanadate (3mM, 30min) whole cell lysate
Predicted MW: multiple
Observed MW: multiple
Exposure time: 30 s
斑点杂交
Dot blot result of Phosphotyrosine Rabbit mAb
Lane 1: Modified peptide library (Phospho-Serine)
Lane 2: Modified peptide library (Phospho-Threonine)
Lane 3: Modified peptide library (Phospho-Tyrosine)
Lane 4: Unmodified peptide library (Serine)
Lane 5: Unmodified peptide library (Threonine)
Lane 6: Unmodified peptide library (Tyrosine)
Primary antibody: Phosphotyrosine Rabbit mAb at 1/500 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫组化
IHC shows positive staining in paraffin-embedded mouse lung (left) and negative staining in phosphatase treated (37 °C, 2h) paraffin-embedded mouse lung (right). Anti- Phospho-Tyrosine antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat lung (left) and negative staining in phosphatase treated (37 °C, 2h) paraffin-embedded mouse lung (right). Anti- Phospho-Tyrosine antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach (left) and negative staining in phosphatase treated (37 °C, 2h) paraffin-embedded mouse lung (right). Anti- Phospho-Tyrosine antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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